四个杂交水稻骨干恢复系的多态性分析  

Polymorphism Analysis for Four Mainly Restore Lines of Hybrid Rice with SSR Markers

作　　者：陈锦文[1,2,3,4,5,6] 朱永生[2,3,4,5,6] 张建福[2,3,4,5,6] 谢华安[2,3,4,5,6] 

机构地区：[1]福建省泉州市农业科学研究所,福建晋江市362212 [2]福建省农业科学院水稻研究所,福建福州350019 [3]农业部华南杂交水稻种质创新与分子育种重点实验室/福州国家水稻改良分中心/福建省作物分子育种工程实验室/福建省水稻分子育种重点实验室,福建福州350003 [4]福建省作物种质创新与分子育种省部共建国家重点实验室培育基地,福建福州350003 [5]水稻国家工程实验室,福建福州350003 [6]杂交水稻国家重点实验室华南基地,福建福州350003

出　　处：《福建农业学报》2014年第4期319-323,共5页Fujian Journal of Agricultural Sciences

基　　金：国家"863"计划重点项目(2006AA100101);福建省科技厅国家科技项目(F2006AA100101);福建省财政专项--福建省农业科学院科技创新团队建设项目(CXTD-1-1301)

摘　　要：选取均匀分布于水稻12条染色体上的134对SSR （Simple Sequence Repeats ，SSR）标记，对明恢63、明恢86、航1号和航2号等4个具有相似遗传背景的水稻骨干恢复系进行遗传多态性分析。结果表明，经航天诱变选育的“航1号、航2号”与原种明恢86之间存在不同程度的遗传多样性。在筛选的134对SSR引物中，具有多态性标记为39对，多态性频率为29.10％。每个标记位点的平均等位基因数为2.10个，变幅为1～4个，平均多态性信息含量指数（PIC ）为0.37。聚类分析结果表明，当以0.87为阀值时可将航天诱变选育的“航1号、航2号”与原种“明恢86”区分开。其中在标记RM234两侧邻近的7个含有重要基因区段的SSR标记中有5个标记存在多态性，表明明恢86在航天诱变过程中可能在具有重要生物学功能的基因座发生了突变，再经系统选育成航1号、航2号优良恢复系，为航天诱变中因发生有利变异而选育新品种提供分子证据。The genetic evolution analysis of four mainly restore lines for hybrid rice including Minghui63 , Minghui86 ,Hang1 and Hang2 with similarly genetic background were carried out in the paper .A total of 134 pairs of simple sequence repeats （SSR） were selected distributed evenly from twelve chromosomes of rice （Oryzae sativa L .） .The results indicated that there were a lot of polymorphic loci between breeding varieties under space flight mutation （Hang1 ,Hang2） and original variety （Minghui86） ,39 polymorphic loci with a polymorphic ratio of＆amp;nbsp;29.10% .The number of alleles per loci ranged from 1 to 4 with an average of 2.1. The value of polymorphism information content （PIC） was 0.37. The four mainly restore lines were classified into 2 groups by clustering .It is distinction between Hang 1 ,Hang2 and Minghui 86 when the threshold value is 0.87. A further analysis on SSR markers distributed in two sides of marker RM 234 which have key gene regions , indicated that there were 5 polymorphism sites from 7 pairs of SSR markers .Bioinformatics analysis showed that those loci have important biological function in Minghui 86 , was mutated under space flight mutation . Furthermore , breeding excellent restoring line Hang 1 and Hang 2 ,provided a molecular evidence that advantageous variations are in space flight mutation for rice breeding .

关 键 词：航天育种 骨干恢复系 SSR 多态性 

分 类 号：S511[农业科学—作物学]