垂花蕙兰丛生芽途径的组织培养技术研究  

In Vitro Micropropagation of Pendent Cymbidiumwith Clustered Shoots

作　　者：周辉明 陈燕[2] 林辉锋 尚伟 王雪凤 

机构地区：[1]福建省三明市农业科学研究院,福建沙县365051 [2]福建省三明市农业局,福建三明365000

出　　处：《福建农业学报》2014年第4期339-344,共6页Fujian Journal of Agricultural Sciences

基　　金：福建省农业科学院"海西农作物品种引种中心区域引种圃建设"项目

摘　　要：以侧芽为外植体,对垂花蕙兰丛生芽途径的组织培养进行研究,结果表明,采用72%乙醇、0.1%升汞和15%次氯酸钠对侧芽进行3次消毒,存活率高达56%,尤其是在春季外植体消毒效果佳;在丛生芽诱导的过程中,6-BA浓度为4.0mg·L-1时,丛芽诱导率可达100%且丛芽分化较多;在丛生芽增殖过程中,6-BA是影响垂花蕙兰丛生芽增殖的重要因素,6-BA 4.0mg·L-1处理增殖系数为3.4,丛生芽较健壮,适合丛生芽增殖;培养基1/2MS+NAA2.0mg·L-1+香蕉泥50g·L-1+AC1g·L-1生根壮苗效果较好;苔藓是垂花蕙兰试管苗移栽较好的基质。Lateral buds were used as the explants for the study on tissue culture of pendent Cymbidium .It showed that by using 72% ethanol ,0.1% mercuric chloride and 15% sodium hypochlorite to disinfect the lateral buds three times ,the bud survival rate could be as high as 56% .The disinfection was especially effective in springtime .The induction rate of the clustered shoots was up to 100% with numerous differentiated buds when 6-BA was applied at the rate of 4.0 mg · L -1 .During the proliferating period of the clustered shoots ,the 6-BA treatment was a key factor that produced the best effect with a proliferating coefficient of clustered shoots of 3.4 and strong buds .For the development in the rooting stage ,on the other hand ,a 1/2 MS medium containing NAA 2.0 mg · L -1 ＋ banana slurry 50 g · L -1 ＋AC 1.0 g · L -1 appeared to be most desirable .And ,moss seemed to be the best base material for the cultivation of the pendent Cymbidium plantlets .

关 键 词：垂花蕙兰 丛生芽 组织培养 

分 类 号：S682.31[农业科学—观赏园艺]