鱼籽酱中盐酸环丙沙星和恩诺沙星高效液相色谱检测方法的建立  

Detection of Ciprofloxacin Hydrochloride and Enrofloxacin in Caviar by HPLC

作　　者：林丽聪[1] 

机构地区：[1]福建省淡水水产研究所,福建福州350002

出　　处：《福建农业学报》2014年第4期389-392,共4页Fujian Journal of Agricultural Sciences

摘　　要：建立了鱼籽酱中盐酸环丙沙星和恩诺沙星的高效液相色谱-荧光检测方法。样品经甲醇及酸化乙腈提取，提取液经氮吹浓缩，残渣用2 mL流动相溶解，正己烷脱脂净化，采用Eclipse XDB-C18色谱柱，以乙腈∶0.02 mol · L -1草酸（14∶86）为流动相，荧光检测器检测。盐酸环丙沙星和恩诺沙星的检出限分别为1、0.5μg · kg -1，在添加量5～120μg · kg -1范围内，平均回收率均大于80％，相对标准偏差均小于5％。说明该方法灵敏、准确，满足常规残留检测要求。A method was developed for the determination of Ciprofloxacin Hydrochloride and Enrofloxacin in Caviar by high performance liquid chromatography with fluorescence detection . The homogenized sample was extracted with methanol and acidified acetonitrile .The extract was concentrated by nitrogen ,and redissolved into 2 mL mobile phase and defatted with n-hexane .Ciprofloxacin Hydrochloride and Enrofloxacin were separated on Eclipse XDB-C18 Column with mobile phases containing acetonitrile and 0.02 mol · L -1 oxalic acid （14 ∶ 86 ） and finally determined by fluorescence Detector .The detection limit of Ciprofloxacin Hydrochloride and Enrofloxacin were 1μg · kg -1 and 0.5 μg · kg -1 , separately .In the range of 5 -120 μg · kg -1 , the mean recoveries were more than 80% . The intraday and interday precisions were less than 5% .It showed that this accurate and sensitive method could be applied for the conventional residue testing requirements .

关 键 词：鱼籽酱 盐酸环丙沙星 恩诺沙星 高效液相色谱法 

分 类 号：S859.84[农业科学—临床兽医学]