CCND基因家族成员分子进化分析  

Molecular Evolution Analysis of CCND Gene Family

作　　者：赵文文[1] 朱婧颖[1] 韩颖颖[1] 

机构地区：[1]上海理工大学医疗器械与食品学院系统生物医学研究中心,上海市200093

出　　处：《医学分子生物学杂志》2014年第3期144-149,共6页Journal of Medical Molecular Biology

基　　金：国家自然科学基金青年基金（No.30900782）,上海市重点学科建设项目（No.S30501）

摘　　要：目的对细胞周期素（cyclinD）家族成员进行分子生物学进化及功能预测，为临床上检测骨质疏松提供更多分子生物学信息。方法对CCND基因家族成员包括CCND1、CCND2和CCND3序列进行分子进化分析，并在所有科属范围和人型总科范围内，计算了同源CCNDs之间的同义和非同义突变频率。结果CCND1和CCND2具有较高的序列相同性，而CCND3的非同义突变率较高，尤其在人型总科各属形成以后，平均每年的同义和非同义突变频率都高于CCND1和CCND2。结论CCND2与CCND1序列相同程度高．而CCND3在序列和进化速率上都显著不同，所以CCND2可以作为骨密度关联研究的候选基因。Objective To provide more molecular biology information for clinical detection of osteoporosis, we have studied the molecular evolution and functional prediction of Cyclin D gene family. Method： Phylogenetic analysis of Cyclin D, including Cyclin D1, Cyclin D2 and Cyclin D3, has been carried out. Meanwhile, synonymous mutation frequency and nonsynonymous mutation frequency among homologous CCNDs from all the studied species were calculated. Results The results showed that CCND1 and CCND2 exhibited higher identity with each other, and high frequency of nonsynonymous mutation occurred in CCND3. In particular, after the divergence of hominoidea, annual average synonymous and nonsynonymous mutation frequencies were higher in CCND3 than in CCND1 and CCND2. Conclusion Our results showed that CCND2 was much identical to CCND1 and CCND3 was significantly different from other two CCNDs based on both sequence identity and molecular evolution rate. And CCND2 can be used as a candidate gene of osteoporosis.

关 键 词：人类细胞周期素D CCND1 CCND2 CCND3 分子进化 

分 类 号：Q343.1[生物学—遗传学]