C／EBPβ在星型胶质细胞中对TGF-β／Smad3通路的调控及分子机制研究  

Regulatory Effect of C/EBPβ on the TGF-β/Smad3 Pathway in Astrocytes

作　　者：陈一鸣[1] 黄承光[1] 

机构地区：[1]第二军医大学附属长征医院神经外科,上海市200433

出　　处：《医学分子生物学杂志》2014年第3期175-178,共4页Journal of Medical Molecular Biology

基　　金：国家自然科学基金（No.81171130）

摘　　要：目的探讨C／EBPβ在星型胶质细胞中对TGF-β／Smad3通路的调控及分子机制。方法采用实时荧光定量PCR和Western印迹的方法分别测定上调星形胶质细胞中C／EBPβ和Smad3的表达对TGF—β及Smad3的影响。并通过上调和沉默细胞中C／EBPβ的表达测定其对细胞增殖及凋亡的影响。结果上调星形胶质细胞中C／EBPβ的表达显著增强了TGF-β及Smad3的表达水平，而上调Smad3的表达则降低了TGF-β的表达；上调星形胶质细胞中C／EBPβ的表达与对照组相比，细胞增殖率提高[（73．26±4．57）％vs（138．29±3．36）％]，凋亡率降低[（24．69±2．06）％vs（6．32±1．63）％]；沉默细胞中C／EBPβ的表达与对照组相比，细胞增殖率降低[（73．26±4．57）％vs（97．54±3．72）％]，凋亡率升高[（24．69±2．06）％vs（14．76±1．43）％]，差异具有统计学意义（P〈0．01）。结论C／EBPβ可通过诱导TGF-β的表达而激活星型胶质细胞中的TGF-β／Smad3信号转导通路，并促进星形胶质细胞增殖。Objective To examine the regulatory effect of C/EBPβ on the TGF-β/Smad3 pathway in astroeytes and the molecular mechanism. Methods Real-time PCR and Western blotting were used to examine the effect of up-regulated expressions of C/EBPβ and Smad3 on TGF-β in astrocytes. The proliferation rate and the apoptosis rate of astrocytes were detected after up-regulation or silencing of the C/EBPβ gene. Results The expression levels of TGF-β and Smad3 were obviously increased in astrocytes overexpressing C/EBPβ, while the expression level of TGF-β was decreased in astroeytes overexpressing Smad3. The proliferation rate was significantly elevated and the apoptosis rate declined in astrocytes with C/EBPβ gene up-regulated [ （73.26 ± 4.57 ）% vs. （138.29± 3.36） % ] for proliferation rate ; [ （24. 69 ± 2. 06） % vs. （6.32 ± 1.63 ） % ] for apoptosis rate, P 〈0. 01 ） . In astrocytes with silencing of C/EBPβ, the proliferation rate [ （73.26 ±4. 57）%vs. （ 97.54 ±3.72） % ] was significantly declined and the apoptosis rate elevated [ （24. 69 ±2. 06 ） % vs. （ 14. 76 ±1.43） %, P 〈 0. 01 ] . Conclusion C/EBPβ can activate the TGF-β/Smad3 pathway in astrocytes and promote the proliferation of astrocytes by inducing the expression of TGF-β.

关 键 词：C/EBPΒ TGF-β/Smad3通路 星型胶质细胞 交通性脑积水 

分 类 号：R349.54[医药卫生—基础医学]