新型固定化乙酰胆碱酯酶传感器的制备及氨基甲酸酯农残检测  

A new biosensor from immobilized acetylcholinesterase to detect carbamate pesticides

作　　者：伍周玲 梁东军[2] 郭明[2] 王晓萌[2] 范文翔[2] 

机构地区：[1]塔里木大学生命科学学院,新疆阿拉尔843300 [2]浙江农林大学理学院,浙江临安311300

出　　处：《浙江农林大学学报》2014年第3期457-464,共8页Journal of Zhejiang A&F University

基　　金：国家自然科学基金资助项目(20877072);浙江省大学生科技创新基金资助项目(2012R412052)

摘　　要：以双醛纤维素(dialdehyde cellulose,DAC)为固载酶基质材料,通过固定化酶化学键联技术制备固定化乙酰胆碱酯酶(DAC-AChE),采用自组装法制备多层壳聚糖/固定化乙酰胆碱酯酶/功能化碳纳米管生物传感器(Chi/DAC-AChE/F-CNTs/GCE),利用交流阻抗法(AC impedance,ACI),循环伏安法(cyclic voltammetry,CV)及差分脉冲伏安法(differential pulse voltammetry,DPV)考察新型传感器对农残的检测性能。结果表明:成功制备了新型固定化乙酰胆碱酯酶电化学传感器;新型传感器对氨基甲酸酯杀虫剂1-萘基-N-甲基氨基甲酸酯(carbary,CAB)具有良好的响应,检测方法响应快速,线性范围宽,灵敏度高,最低检出限可达3.5μg·L-1,具有很好的重现性和稳定性,有关结果可为氨基甲酸酯类杀虫剂的检测提供一种新的分析方法。Dialdehyde cellulose （DAC）,an immobilized enzyme matrix material,was used to prepare an immobilized acetylcholinesterase according to immobilized enzyme chemical linking technology.The immobilized acetylcholinesterase was then used to prepare a new multilayer chitosan / immobilized acetylcholinesterase/ functionalized carbon nanotube / glassy carbon electrode （Chi/DAC-AChE/F-CNTs/GCE） biosensor.AC impedance （ACI）,cyclic voltammetry （CV）,and differential pulse voltammetry （DPV） were utilized to characterize the detection performance of pesticides acting on the biosensor.Results showed that the new Chi/DAC-AChE/FCNTs/GCE biosensor was successfully prepared and had a good response to carbaryl （CAB）.This new sensor provided a quick and easy detection method with a wide range and a high sensitivity having a minimum detection limit of 3.5 μg ·L-1 with good reproducibility and stability.These results could provide a new analytical method for the detection of carbamate pesticides.

关 键 词：双醛纤维素 乙酰胆碱酯酶 生物传感器 氨基甲酸酯类杀虫剂 农药残留 检测 

分 类 号：S481.8[农业科学—农药学]