水稻OsNHO1基因cDNA序列分离及表达分析  

Isolation and Expression Profiling of Rice OsNHO1 Full-length cDNA

作　　者：高志亮[1,2] 肖晓蓉[1,2] 陈艳[3] 黎秀琼[1,2] 符秀梅[1,2] 林道哲[1,2] 陈银华[1,2] 

机构地区：[1]海南大学热带生物资源可持续利用重点实验室,海南海口570228 [2]海南大学农学院,海南海口570228 [3]北京农学院,北京102206

出　　处：《热带生物学报》2014年第1期30-35,共6页Journal of Tropical Biology

基　　金：转基因生物新品种培育重大专项(2009ZX08009-41B);国家自然科学基金(31260345);海南省重大科技项目(ZDZX2013023-2);公益性行业科研专项(201403075)

摘　　要：OsNHO1是一种甘油激酶,受多种因素的诱导,在植物先天免疫反应中起着重要的作用。笔者采用同源序列法,根据已报道的拟南芥NHO1基因序列结合水稻基因组测序结果,筛选水稻OsNHO1基因,采用RT-PCR获得水稻OsNHO1基因全长cDNA(1 590 bp,529AA),并通过半定量RT-PCR方法分析OsNHO1基因在SA,PXO99刺激下的表达模式,结果显示OsNHO1基因的表达受SA,PXO99的诱导,为进一步研究OsNHO1的具体功能和作用机理奠定了基础。Arabidopsis non-host resistance gene （AtNHO1） encodes a putative glycerol kinase and plays an important role in plant PAMPs triggered immunity （PTI）.The full length cDNA of rice （Oryza sativa） gene OsNHO1 was isolated by reverse transcription PCR,and the sequencing result showed that the open reading frame of OsNHO1 is 1 590 bp,encoding a 529 amino acids with a conserved NBD-sugar-kinase domain similar with NHO1 from Arabidopsis.Bioinformatics analysis showed that the amino acid sequence of the OsNHO1 is 99%similar with that of the NHO1 from Arabidopsis thaliana.Semi-quantitative RT-PCR analysis of the expression of OsNHO1 induced by SA and PXO999 showed that the OsNHO1 gene expression was induced by SA,PXO99,indicating that OsNHO1 is related to SA-mediated signaling transduction pathway and plays a key role in rice PAMPs triggered immunity （PTI）.

关 键 词：白叶枯病 植物先天免疫反应 非寄主抗性 OsNHO1基因 半定量RT-PCR 

分 类 号：Q943.2[生物学—植物学] S511[农业科学—作物学]