长白猪和大白猪生长激素基因多态性及其对部分经济性状的影响  

Analysis on Polymorphism of GH Gene and Its Association with some Economic Traits in Landrace and Yorkshire Pigs

作　　者：王靖[1] 刘颖[1] 董文华[1] 程洪青 包文斌[1,3] 吴圣龙[1,3] 

机构地区：[1]扬州大学动物科学与技术学院江苏省动物遗传繁育与分子设计重点实验室,江苏扬州225009 [2]扬州创日畜牧科技有限公司,江苏扬州225009 [3]扬州市种猪繁育工程技术研究中心,江苏扬州225009

出　　处：《中国畜牧杂志》2014年第11期1-4,共4页Chinese Journal of Animal Science

基　　金：国家星火计划重点项目(2012GA690003);江苏省科技支撑计划(农业)(BE2010371、BE2012330);扬州市农业科技攻关项目(YZ2011048、YZ2011153);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：本研究采用PCR-RFLP方法检测长白猪和大白猪GH基因+146^+652 bp序列Apa I酶切位点的多态性,并进一步分析该多态位点对达100 kg日龄、眼肌厚、背膘厚及第1~4胎次繁殖性能等部分经济性状的影响。结果表明:长白猪和大白猪GH基因+146^+652 bp序列Apa I酶切位点均检测到AA、AB和BB 3种基因型,2个群体在此位点均处于Hardy-Weinberg平衡状态。在长白猪中,AA基因型个体的背膘厚显著高于AB和BB基因型个体(P<0.05),第1~4胎次总产仔数和产活仔数总体上符合AB>BB>AA的趋势;在大白猪中,AA基因型个体的眼肌厚显著低于AB和BB基因型个体(P<0.05),第1~4胎次总产仔数和产活仔数总体上符合BB>AB>AA的趋势。本研究结果初步说明,AB、BB基因型个体具有较高的瘦肉率和繁殖性能,对所检测的部分经济性状而言,AA基因型为不利基因型。The polymorphism of the Apa Ⅰ restriction site in growth hormone （GH） gene ＋146～＋652 bp fragments of Landrace and Yorkshire pigs were detected with PCR-RFLP method and the relationship between days geting 100 kg,corrected back-fat thickness,loineye depth,number born and number born alive in 1～4 litters and different genotypes.The results showed that the Apa Ⅰ restriction site in GH gene ＋146～＋652 bp fragments of Landrace and Yorkshire pigs could be divided into AA,AB and BB genotypes and the pattern types of the restriction site were fit with Hardy-Weinberg equilibrium.In the Landrace,the corrected back-fat thickness AA genotype were significantly higher than that with AB and BB genotype （P＜0.05）,and the number born and number born alive in 1 ～4 parities with different genotypes had the same trend：AB ＞BB ＞AA; while in Yorkshire the loineye depth with AA genotype were significantly lower than that with AB and BB genotype （P＜0.05）; and the trend of the number born and number born alive in 1～4 parities was BB＞AB＞AA.It was concluded that the lean meat percentage and reproductive performances with AB and BB genotype were relative high and AA genotype was the unfavorable genotype for these economic traits measuring in this study.

关 键 词：GH基因 PCR-RFLP 经济性状 猪 

分 类 号：S828.2[农业科学—畜牧学]