天冬氨酸对脂多糖刺激断奶仔猪肠道形态结构和黏膜抗氧化能力的影响  

Effect of Aspartate on Antioxidant Capacity and Structure of Intestinal Mucosa of Weaned Piglets Challenged with Lipopolysaccharide

作　　者：冷炜博[1] 刘玉兰[1] 李爽[1] 石海峰[1] 朱惠玲[1] 侯永清[1] 皮定安[1] 

机构地区：[1]武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉430023

出　　处：《中国畜牧杂志》2014年第11期32-36,共5页Chinese Journal of Animal Science

基　　金：教育部新世纪优秀人才支持计划(NCET-10-0158);国家自然科学基金面上项目(31172222;31372318);湖北省自然科学基金重点项目(2010CDA050);湖北省国际科技合作项目(2011BFA008)

摘　　要：本试验旨在研究天冬氨酸(ASP)对脂多糖(LPS)刺激断奶仔猪肠道结构和黏膜抗氧化能力的影响。选取(21±1)d日龄断奶、平均体重(7.37±0.04)kg的杜×长×大三元杂交仔猪24头,随机分为对照组(基础日粮)、LPS组(基础日粮+LPS)、0.5%ASP组(基础日粮+0.5%ASP+LPS)、1.0%ASP组(基础日粮+1.0%ASP+LPS)4组,试验期24 d。试验第24天,分别给3个试验组的猪注射100μg/kg体重的LPS,对照组注射等量的生理盐水。注射后24 h屠宰,取小肠样品待测。结果表明:0.5%或1.0%ASP可显著缓解LPS刺激导致的空肠绒毛高度降低、隐窝深度升高和绒毛高度/隐窝深度的降低,增加回肠的绒毛高度、绒毛高度/隐窝深度(P<0.05);0.5%ASP对空肠超氧化物歧化酶、回肠谷胱甘肽过氧化物酶活性有提高的趋势(P<0.10);0.5%或1.0%ASP显著降低了空肠和回肠丙二醛含量(P<0.05)。以上研究结果显示,ASP有提高仔猪肠黏膜抗氧化相关酶的酶活趋势,降低过氧化物的产生,从而改善肠道形态结构。The study was conducted to investigate the effect of aspartate （ASP） on antioxidant capacity of intestinal mucosa of weaned piglets challenged with lipopolysaccharide （LPS）.Twenty-four weaned piglets （Duroc （Landrace （Large） weighing （7.37±0.04） kg were randomly assigned into 4 groups：control （basal diet）; LPS （basal diet＋LPS）;0.5％ ASP （basal diet＋0.5％ ASP＋LPS）; 1.0％ ASP （basal diet＋1.0％ ASP＋LPS）.On day 24,the piglets in the LPS group,0.5％ ASP group and 1.0％ ASP group were injected intraperitoneally with 100 μg/kg BW LPS,and the piglets in the control group were injected with the same dose of physiological saline.After 24 h saline or LPS injection,all pigs were sacrificed to obtain intestinal mucosa for analysis.The results showed that 0.5％ or 1.0％ ASP alleviated the decrease of jejunal villus height and villus height/crypt depth and the increase of crypt depth induced by LPS,and increased ileal villus height and villus height/crypt depth （P＜0.05）.0.5％ ASP had a tendency to improve the activities of superoxide dismutase in jejunum and glutathione peroxidise in ileum （P＜0.10）; 0.5％ or 1.0％ ASP significantly reduced the content of malondialdehyde in jejunum and ileum （P＜0.05）.In conclusion,ASP has tended to improve the activities of antioxidant enzymes,and alleviates histological intestinal injury induced by LPS challenge in piglets.

关 键 词：天冬氨酸 脂多糖 肠黏膜 抗氧化 断奶仔猪 

分 类 号：S828.5[农业科学—畜牧学]