电针内关穴对糖尿病心肌病大鼠线粒体Ca2＋转运及心肌细胞凋亡效应的影响  

Analysis of electroacupuncturing Neiguan points on mitochondrial Ca2＋ release and cardiomyocyte apoptosis of diabetic cardiomyopathy in rats

作　　者：肖凤英[1] 崔金涛[1] 张压西[1] 贺劲[1] 李敏[1] 张军[1] 

机构地区：[1]湖北省武汉市中医医院内分泌科,430014

出　　处：《国际中医中药杂志》2014年第6期544-546,共3页International Journal of Traditional Chinese Medicine

基　　金：本课题为武汉市卫生局项目（项目编号：WZ11C06）

摘　　要：目的：探讨电针内关穴对糖尿病心肌病大鼠线粒体Ca2＋转运及心肌细胞凋亡效应的影响。方法 Wistar大鼠雄性30只，按照随机数字表法将大鼠随机分为正常组、模型组和治疗组各10只，采用一次性腹腔注射链脲佐菌素（STZ）建立糖尿病心肌病大鼠模型。治疗组电针内关穴治疗8周后，迅速取出心肌，提取线粒体，采用双光束紫外分光光度仪通过测试Ca2＋指示剂AⅢ吸光值检测Ca2＋转运，心肌细胞凋亡指数检测采用TUNEL法测定。结果与模型组线粒体Ca2＋转运检测吸光值（0.077±0.025）、心肌细胞凋亡指数（0.53±0.04）比较，治疗组[Ca2＋转运（0.051±0.014）、心肌细胞凋亡指数（0.49±0.36）]明显降低（P＜0.05）。结论电针内关穴可降低糖尿病心肌病大鼠心肌细胞凋亡指数，可能与其降低线粒体Ca2＋内流有关。Objective To investigate the influence of electroacupuncturing Neiguan points on mitochondrial Ca2＋release and cardiomyocyte apoptosis of diabetic cardiomyopathy in rats. Methods 30 Wistar rats were randomly divided into a control group, a model group and a treatment group（10 in each group）. In the treatment group, after eight weeks electroacupuncture Neiguan point treating diabetic cardiomyopathy in rats, the myocardium was quickly removed,mitochondrial was extracted,dual-beam UV spectrophotometer was adopted to detecting Ca2＋transportation by testing Ca2＋indicator A Ⅲ absorbance, and Cardiomyocyte apoptosis was evaluated by terminal-deoxynucleotidyl transferase mediated deoxy-UTP nick end labeling （TUNEL）. Results Ca2＋ indicator A Ⅲ absorbance detection（0.051±0.014） and cardiomyocyte apoptosis（0.49±0.36）were more depressed in the treatment group than in the model group[（0.077±0.025）, （0.53±0.04）], there were significantly different（P〈0.05） Conclusion Effect of Electroacupuncturing Neiguan points decreased myocardial apoptosis index in rats with diabetic cardiomyopathy, which may be related to the reduction of mitochondrial Ca2＋influx.

关 键 词：大鼠 糖尿病心肌病 内关穴 线粒体Ca2+转运 心肌细胞凋亡 

分 类 号：R245[医药卫生—针灸推拿学]