四妙勇安汤对缺氧人脐静脉内皮细胞炎症因子白细胞介素-6、肿瘤坏死因子-α及血管内皮生长因子的影响  

Investigation of Simiaoyong'an decoction on IL-6,TNF-α and VEGF in human umbilical vein endothelial cells with anoxia

作　　者：高慧[1] 刘真[1] 于慧卿[1] 付达[1] 郭娜[1] 

机构地区：[1]河北省石家庄市中医院心血管内科,河北石家庄050051

出　　处：《河北中医》2014年第6期898-900,906,共4页Hebei Journal of Traditional Chinese Medicine

摘　　要：目的观察四妙勇安汤对缺氧人脐静脉内皮细胞(HUVEC)炎症因子白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)及血管内皮生长因子(VEGF)水平的影响。方法将HUVEC细胞株进行传代培养后,将实验用细胞分为3组,正常组、模型组、四妙勇安汤组。正常组将正常的HUVEC细胞加入正常培养液培养;模型组将HUVEC细胞进行缺氧处理后加入正常培养液培养;四妙勇安汤组将HUVEC细胞进行缺氧处理后加入含10%四妙勇安汤醇沉药液的细胞培养液培养。用酶联免疫吸附法(ELISA)测定各组IL-6、TNF-α水平,用免疫组化法检测各组VEGF生成量的表达。结果模型组、四妙勇安汤组IL-6、TNF-α及VEGF水平与正常组比较差异均有统计学意义(P<0.01),IL-6、TNF-α上升,VEGF下降;但四妙勇安汤组IL-6、TNF-α上升水平及VEGF下降水平均小于模型组(P<0.01),四妙勇安汤可以改善缺氧HUVEC细胞中IL-6、TNF-α及VEGF水平变化。结论四妙勇安汤对缺氧HUVEC细胞能有效地抑制炎性因子的分泌,促进内皮细胞的增殖,具有抗炎及促进血管新生的作用,对损伤的血管内皮细胞具有一定的保护作用。Objective To investigate the effect of Simiaoyong＇an decoction on IL-6, TNF-αand VEGF in human umbilical vein endothelial cells （HUVEC） with anoxia.Methods Human umbilical vein endothelial （HU-VEC） cell lines were subcultured .The cells were divided into three experimental groups , the normal group , model group, Simiaoyong＇an decoction group .The normal HUVEC in the normal group were cultured in normal culture solu-tion.HUVEC in model group received anaerobic treatment and then were cultured by normal culture solution .HUVEC in Simiaoyong＇an decoction group received anaerobic treatment and then were cultured by 10% Simiaoyong＇an decoc-tion culture solution.The levels of IL-6, TNF-a and VEGF were measured by ELISA and immunohistochemistry in each group .Results There were significant differences between model group , Simiaoyong＇an decoction group and the normal group on the levels of IL -6, TNF-a and VEGF （ P〈0.01）.The levels of IL -6 and TNF -αwas in-creased , and VEGF was decreased .But the increase of IL -6 and TNF-αand the decrease of VEGF in Simiaoyong＇an decoction group was less than that in model group （P〈0.01）.Simiaoyong＇an decoction can improve the levels of IL-6, TNF-a and VEGF in HUVEC with anoxia .Conclusion Simiaoyong＇an decoction can effectively inhibit the se-cretion of inflammatory factors in human umbilical vein endothelial cells with anoxia , promote the proliferation of en-dothelial cells , have anti -inflammatory and angiogenic effects on vascular endothelial cell injury has a protective effect.

关 键 词：四妙勇安汤 脐静脉 内皮细胞 缺氧 炎症趋化因子类 

分 类 号：R285.5[医药卫生—中药学]