Slit2蛋白对深静脉血栓作用的实验研究  

Experimental study on effect of Slit2 protein on deep vein thrombosis in mice

作　　者：勾红菊[1] 王丽京[2] 兰天[2] 吴腾[2] 吴平香[1] 顾取良[3] 郑凌云 温定文[2] 丁彦青[1] 

机构地区：[1]南方医科大学基础医学院,广州510515 [2]广东药学院血管生物研究所,广州510006 [3]广东药学院基础学院,广州510006

出　　处：《重庆医学》2014年第16期2016-2018,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目(31271455;81200308;31100852;31200861)

摘　　要：目的探讨Slit2过表达对深静脉血栓形成的影响。方法利用Slit2过表达转基因小鼠进行下腔静脉结扎造模,观察血栓形成的情况,测量出血时间、血栓重量长度比,HE染色观察血栓形成的病理形态,并同时设立C57小鼠对照组。结果成功建立了小鼠下腔静脉血栓模型;在造模48h后Slit2过表达转基因小鼠与C57小鼠相比,裸血栓重量长度比明显减小;出血时间延长;Slit2过表达小鼠形成的血栓中白色血栓所占比例较小,而C57小鼠则白色血栓所占比例较大。结论 Slit2过表达能通过抑制白色血栓形成,从而有效抑制深静脉血栓的形成。Objective To investigate the effect of Slit2 overexpression on deep vein thrombosis in mice.Methods Slit2 overex-pression transgenic mice were adopted to establish the model of deep vein thrombosis by ligating venae cava inferior.The thrombo-sis situation was observed.The bleeding time and the ratio of thrombus weight to length were measured.The histopathological fea-tures of thrombogenesis were observed by the HE staining.At the same time the C57 mice control group was set up.Results The thrombus model of mouse venae cava inferior was successfully established;compared with the C5 7 mice,the ratio of naked thrombus weight to length after 24 h of the constructing model in the Slit2 overexpression transgenic mice was significantly decreased;the bleeding time was prolonged;the proportion of the white thrombi to the formative thrombi in the Slit2 overexpression mice was very small,while which in the C5 7 mice was larger.Conclusion Slit2 overexpression can effectively inhibit the deep vein thrombosis through inhibiting the white thrombosis.

关 键 词：深静脉血栓形式 SLIT2 基因工程小鼠 

分 类 号：R543.6[医药卫生—心血管疾病]