正交试验优选防己中粉防己碱和防己诺林碱的提取工艺  

Optimization of Extraction Process of Fangchinoline and Tetrandrine in Stephaniatetrandra S.Moore by Orthogonal Experiment

作　　者：张建岭[1] 张路[1] 李士栋[1] 张淹[1] 胡永水[1] 

机构地区：[1]国家胶类中药工程技术研究中心,山东东阿阿胶股份有限公司,山东聊城252201

出　　处：《中国药物评价》2014年第2期79-81,85,共4页Chinese Journal of Drug Evaluation

基　　金："重大新药创制"科技重大专项(编号:2011ZX09201-201-10)资助项目;山东省科技发展计划--经典中药大品种阿胶技术升级;编号:2013G0031901

摘　　要：目的:优选防己中粉防己碱和防己诺林碱的最佳提取工艺。方法:以总生物碱的含量和浸膏的率为考察指标,采用正交试验,考察提取溶媒的浓度、提取时间、提取次数和提取溶媒的用量对提取的影响,确定了防己中粉防己碱和防己诺林碱的最佳提取工艺。结果:最佳提取工艺条件是:以6倍量60%乙醇提取3次,每次1小时。结论:优选得到的提取工艺得率高,操作简便易行。Objective： To optimize the extraction process of Fangchinoline and Tetrandrine in StephaniaTetrandra S. Moore by orthogonal design. Methods： The optimal extraction conditions,such as the concentration of extraction solvent,extraction time,extraction times and amount of solvent to material were investigated by orthogonal design; the content of the total alkaloids and Extract yield were the inspection index. Results： The optimal conditions for the extraction process were as follows,and took 60% alcohol as dissolving medium,extract 3 times,1 h / time. Conclusion： The extraction technology developed after optimization was a high efficiency and easier to operate.

关 键 词：正交试验 粉防己碱 防己诺林碱 防己 

分 类 号：R284.2[医药卫生—中药学]