不同地区竹叶中多糖的提取测定  

Determination of Polysaccharides from Bamboo Leaves in Different Regions

作　　者：喻谨 岳永德 汤锋 姚曦 王坤 

机构地区：[1]国际竹藤中心,北京100102

出　　处：《生物质化学工程》2014年第3期40-43,共4页Biomass Chemical Engineering

基　　金："十二五"国家科技支撑计划资助(2012BAD23B03)

摘　　要：采用苯酚-硫酸法测定了10种竹叶多糖的含量,分析比较了不同竹种竹叶多糖含量的差异;利用离子色谱仪测定了竹叶多糖的组成。结果表明：10种竹叶含多糖0.18%~0.93%,最高为福建青秆竹,最低是长宁佛肚竹;同一地区不同竹种的竹叶多糖含量存在极显著差异。竹叶多糖由5种单糖组成,分别是阿拉伯糖、半乳糖、葡萄糖、木糖和甘露糖,其中阿拉伯糖占18.32%~36.63%,半乳糖占19.16%~38.19%,葡萄糖占16.90%~54.45%,木糖占5.88%~16.32%,甘露糖占1.53%~10.89%。The content of polysaccharides from ten species of bamboo leaves was determined by phenol-sulfuric acid method.The content difference among the different bamboo leaves was analyzed.The monosaccharide composition of polysaccharides from ten species of bamboo leaves was studied using high performance anion exchange chromatography（ HPAEC） respectively.The results indicated that the content range of polysaccharides was 0.18% to 0.93%,the highest was Bambusa tuloides Munro in Fujian,and the lowest was Bambusa ventricosa McClure in Changning.Significant differences were found among the content of polysaccharides from different species of bamboo leaves in the same area.Bamboo polysaccharides in different species were composed of arabinose 18.32%-36.63%,galactose 19.16%-38.19%,glucose 16.90%-54.45%,xylose 5.88%-16.32%,and mannose 1.53%-10.89%,respectively.

关 键 词：竹叶 多糖 含量 单糖组成 

分 类 号：R284.2[医药卫生—中药学]