低剂量内毒素诱导构建子痫前期大鼠模型  

Establishment of rat model with preeclampsia induced by low-dose lipopolysaccharides

作　　者：李静[1] 唐卉[1] 

机构地区：[1]广西医科大学第一附属医院,南宁530021

出　　处：《山东医药》2014年第19期26-28,共3页Shandong Medical Journal

基　　金：广西壮族自治区自然科学基金资助项目(桂科自0447070)

摘　　要：目的用低剂量内毒素诱导构建子痫前期大鼠模型。方法将12只妊娠大鼠随机分为观察组6只和对照组6只,于妊娠第14天,观察组尾静脉缓慢注射2 mL内毒素(1μg/kg),对照组注射2 mL生理盐水。测量两组大鼠妊娠第7、13、15、17、19天时的血压,采用免疫比浊法测定两组大鼠妊娠第12、15天的24 h尿蛋白。妊娠第20天大鼠均未分娩,对其进行剖宫取胎,观察仔鼠情况,并取胎盘组织做HE染色,观察病理形态学改变。结果妊娠第15、17、19天时观察组大鼠收缩压均明显升高(高于基础值30 mmHg),对照组血压无明显变化,两组比较,P均<0.05。妊娠第15天,观察组24 h尿蛋白(高于基础值2倍)为(6.07±0.75)mg/24 h,对照组为(1.90±0.43)mg/24 h,两组比较,P<0.05。观察组组织病理学检查可见基带炎性细胞浸润、迷路带组织核碎裂、纤维蛋白沉积等胎盘炎性改变。结论低剂量内毒素能诱导大鼠产生高血压、蛋白尿、胎盘炎性改变等子痫前期表现,子痫前期大鼠模型构建成功。Objective To induce the rats to copy the rat model with preeclampsia by using low-dose lipopolysacchar-ides.Methods：twelve pregnant rats were randomly divided into 2 groups： experimental group and normal control group , with 6 rats in each group.The experimental group were injected with 2ml of lipopolysaccharides solution of 1μg/kg through tail veins on day 14 of pregnancy, while the control group were injected with 2ml saline.The blood pressure on day 7,13, 15,17,19 were monitored, and the 24-hour proteinuria on day 12,15 were measured by Immune turbidimetry .All the rats had no delivery on day 20, and then the uterine cesarean delivery were made to them , and observed the status of the new-born mouse, and got the placentas to make HE staining and observed the pathological changes .Results：The systolic pres-sure of the experimental group rose significantly on day 15,17 and 19 of pregnancy, which was higher more than 30 mmHg of the basic pressure .The blood pressure of the normal control group had no obvious change .The P of both two groups were less than 0.05 while they compared with each other .On day 15 of pregnancy, the 24-hour proteinuria（more than twice as much as the basic pressure） of the experimental group was（6.07 ±0.75）mg/24h, while that of the normal group was （1. 90 ±0.43）mg/24h, and P〈0.05 when these 2 groups compared with each other .The experimental group showed placen-tal inflammatory changes such as baseband inflammatory cell infiltration , labyrinth zone tissue nuclear fragmentation and fi-brin deposition by examination of the pathology .Conclusion：low-dose lipopolysaccharides could induce preeclampsia per-formances such as hypertension , proteinuria and placental inflammatory changes in rats .The The rat model with preeclamp-sia was successfully established .

关 键 词：妊娠并发症 子痫前期 动物模型 内毒素 

分 类 号：R714.245[医药卫生—妇产科学]