孕哺期暴露壬基酚对雄性仔鼠脑皮质肿瘤坏死因子-α表达的影响  

Influence of exposure to p-nonylphenol during pregnancy on TNF-α in the cortex of male rat pups

作　　者：张恒[1] 俞捷[2] 岳欢[3] 罗娅[2] 封校亚 许洁[2] 

机构地区：[1]遵义市第一人民医院病理科,贵州遵义563000 [2]遵义医学院公共卫生学院,贵州遵义563000 [3]遵义市第一人民医院检验科,贵州遵义563000

出　　处：《现代医药卫生》2014年第11期1601-1602,共2页Journal of Modern Medicine & Health

基　　金：国家自然科学基金(201381360439);贵州省教育厅青年基金(黔教合KY字[2013]198);贵州省卫生厅基金(gzwkj20131127);遵义医学院博士启动基金(ZMKD2012-002);遵义医学院大学生创新基金(20136509)

摘　　要：目的观察母鼠孕期和哺乳期暴露壬基酚(NP)对其雄性仔鼠脑皮质肿瘤坏死因子-α(TNF-α)表达的影响。方法根据妊娠时间将32只孕鼠随机分为对照组及NP低、中、高剂量暴露组,每组各8只;消化道暴露剂量分别为0、25、50、100 mg/(kg·d);暴露时间为受孕第6天到出生后21 d(仔鼠哺乳结束)。采用免疫组织化学方法检测仔鼠出生后21、60 d脑皮质TNF-α表达水平,分析其表达水平与NP暴露之间的关系。结果与对照组比较,NP低、中、高剂量暴露组仔鼠出生后21、60 d TNF-α表达水平增高。结论 NP孕期暴露使仔鼠脑皮质TNF-α表达水平增加,炎性反应可能是NP引起仔鼠脑皮质损伤的机制。Objective To observe the influence of exposure to p-nonylphenol（NP） during pregnancy and lactation on tumor necrosis factor（TNF）-αin the cortex of male rat pups. Methods According to the pregnancy time,32 pregnant rats were randomly divided into the control group and the exposure group （of low,middle,high dose）. Exposure dosages of digestive tract were 0,25,50,100 mg/（kg·d） respectively. Exposure duration was from 6th day of conception to the 21st day after birth （end of the lactation for newborn rats）. Immunohistochemistry was adopted to determine the expression level of TNF-αin the cortex of male rats pups on the 21st day and 60th day after birth ,and the association between NP exposure and these alterations in TNF-αwas analyzed as well. Results Compared with the control group,the expression level of TNF-αin exposure group（of low,middle,high dose） increased on the 21st day and 60th day after birth. Conclusion Exposure to NP during gestation might induce an increase of TNF-αexpression levels in the cortex of pup rats,and the inflammatory reaction might damage mechanism of the cortex of pup rats.

关 键 词：酚类 肿瘤坏死因子α 大脑皮质 妊娠 动物 大鼠 Sprague-Dawley 壬基酚 仔鼠 

分 类 号：R587.1[医药卫生—内分泌]