不同术式子宫切除对卵巢组织的影响  

The influence of different operative methods hysterectomy for ovarian tissue

作　　者：李俐辉[1] 李洁[2] 米梅艳[1] 张红娟[1] 

机构地区：[1]河北省石家庄市第四医院,050011 [2]河北省人民医院

出　　处：《河北医药》2014年第12期1772-1774,共3页Hebei Medical Journal

基　　金：河北省人口和计划生育委员会科学技术研究计划项目(编号:2012-A20)

摘　　要：目的通过建立不同术式子宫切除的动物模型,探讨不同范围子宫切除术对卵巢细胞的影响,为临床选择提供恰当的术式。方法将15只成年雌性同龄新西兰兔,随机分为全子宫切除组、三角形子宫切除组和对照组,每组5只。行不同范围子宫切除术,4个月取材,流式细胞仪检测卵巢细胞凋亡率,光镜下观察卵巢组织及卵泡发育情况。结果三角形子宫切出组与对照组卵巢组织及卵泡发育无明显变化,子宫全切组卵巢细胞凋亡率、卵巢组织及卵泡发育退化显著(P<0.05)。结论三角形子宫切除术对卵巢的不良影响优于子宫全切组。Objective Through the establishment of animal model of different operative methods of hysterectomy ,to investigate the impact of different range hysterectomy on ovarian cells in order to provide proper operative methods for clinical choice.Methods The 15 adult female New Zealand rabbits were randomly divided into three groups：whole hysterectomy group,triangularis hysterectomy group ,control group,with 5 rabbits in each group.After 4 months,the specimens of ovary tissue were obtained , ovarian cell apoptosis rate was detected by flow cytometry and the developmental condition of ovarian tissue and follicles was observed under light microscope .Results There was no significant difference in the developmental condition of ovarian tissue and follicles between triangularis hysterectomy group and control group ,however,the total number of ovarian follicle in ovarian cortex in whole hysterectomy group was obviously decreased ,and growing follicle at different levels was decreased ,especially mature follicle , so was corpus luteum quantity .There was a significant difference in ovarian cell apoptosis rate between whole hysterectomy group and triangularis hysterectomy group as well as control group （ P 〈0.05）. However there was no significant difference in ovarian cell apoptosis rate between triangularis hysterectomy group and control group （ P 〉0 .05 ） .Conclusion Triangle hysterectomy is superior to whole hysterectomy , for the former has less adverse effects on ovary than the latter has .

关 键 词：子宫切除 卵巢 卵泡 凋亡率 

分 类 号：R713.42[医药卫生—妇产科学]