玄参提取物对小鼠急性化学性肝损伤保护作用的研究  

Hepatoprotective Effects of the Extracts from Scrophularia ningpoensis Hemsl.on Acute Liver Injury of Mice

作　　者：吴亚辉[1] 陈志鹃[1] 杨玲玲[1] 冯成[1] 龙霞[1] 高平[1] 

机构地区：[1]四川大学生命科学学院,生物资源与生态环境教育部重点实验室,成都610065

出　　处：《四川动物》2014年第3期386-389,共4页Sichuan Journal of Zoology

摘　　要：采用四氯化碳(CCl4)腹腔注射构建小鼠急性肝损伤模型;利用生物活性跟踪法,以小鼠血清中谷丙转氨酶(ALT)活性和谷草转氨酶(AST)活性为检测指标,从玄参根提取物筛选保肝活性的物质。结果表明乙酸乙酯萃取部位保肝效果最好,能够显著降低血清ALT和AST活性(P<0.01),其次为正丁醇相。对活性较好的乙酸乙酯部位进一步通过硅胶柱层析分离,结果表明段F02和段F03能显著降低血清ALT和AST活性(P<0.05或P<0.01),降低肝脏MDA含量(P<0.05或P<0.01),增强肝脏SOD活力(P<0.05或P<0.01),提示玄参提取物可能通过抑制脂质过氧化发挥保肝作用。Mice were injected with carbon tetrachloride （CCl4） to establish acute liver injured model. The hepatoprotective components of the roots of Scrophularia ningpoenis Hemsl. were screened by bioactive tracking method by testing the ac, tivities of serum enzyme glutamic-pyruvic transaminase （ALT） and glutamic oxalacetie transaminase （AST） on acute liver injury mice. The result showed that the best hepatoprotective effect of the extracts was ethyl acetate, followed by normal butanol phase. Ethyl acetate could significantly decrease serum ALT and AST （ P 〈 0.01 ）. Silica gel column chromatography was used to separate active ingredients from ethyl acetate. Fraction F02 and F03 could significantly decrease sermn ALT （ P 〈 0.05 ） and AST activities （ P 〈 0.05 or P 〈 0.01 ）, live MDA content （P 〈 0.05 or P 〈 0.01 ）, but increase liver SOD activity （ P 〈 0.05 or P 〈 0.01 ）. These results indicated that the hepatoprotective effects of the extracts from Scrophularia ningpoensis Hemsl. may be related to anti-oxidative effect for lipids.

关 键 词：玄参 保肝活性 生物活性跟踪法 柱层析 

分 类 号：R285.5[医药卫生—中药学]