某林麝场林麝感染假结核耶尔森菌的诊治  

Diagnosis and Treatment of Forest Musk Deer Infected with Yersinia pseudotuberculosis

作　　者：解冰冰[1] 彭家兰 钟志军[1] 彭广能[1] 彭西[1] 杨利平 周潇潇[1] 李刚诗[1] 刘学涵[1] 石锦江[1] 陈英 杨平 李兵 

机构地区：[1]四川农业大学动物医学院,动物疫病与人类健康四川省重点实验室,四川雅安625014 [2]重庆市石柱县西沱兽医站,重庆409106 [3]雅安市名山区农业局,四川雅安625100 [4]重庆市中关动物药业有限公司,重庆404300

出　　处：《四川动物》2014年第3期419-424,433,共7页Sichuan Journal of Zoology

基　　金：国家自然科学基金面上项目(31272620);教育部“长江学者与创新团队发展规划”创新团队项目(IRT0848)

摘　　要：为寻求四川宝兴某家养林麝死亡的原因及控制措施,采集一例病死林麝各组织器官进行病理切片观察和细菌分离,对分离到的病菌进行形态学观察、16S rDNA序列分析、小鼠致死试验、生化鉴定及药敏试验。结果显示,切片中各组织含有大量细菌团;从肠系膜淋巴结、肝、脾、肾和肺中只分离到1株病菌;16S rDNA测序分析发现该菌与耶尔森菌16S基因的相似性高达100%,生化及鉴别试验最终判定为假结核耶尔森菌。该菌对头孢噻肟、头孢西丁、丁胺卡那霉素等药物敏感。结果证实,此次林麝死亡的原因为假结核耶尔森菌引起的败血症,并及时应用敏感抗生素控制了病情。In order to explore the causes and treatment of acute death of captive forest musk deer at Baoxing county, Sichuan province, one sample was collected for pathological observation and bacteria isolation. The isolated bacteria were conducted to morphology observation, 16S rDNA sequences analysis, biochemical identification, mice pathogenic experiment and drug susceptibility test. The results showed that the pathological slices of internal organs including intestinal lymph nodes, liver, spleen, kidney and lungs contained a lot of bacteria groups. The result of 16S rDNA sequencing analysis showed that the similarity of 16S gene between the isolated strain and Yersinia strain was 100%. And the biochemical test confirmed that the isolated strain was Yersinia pseudotuberculosis. The strain was sensitive to cefotaxime, cefoxitin and ami- kacin, etc. The results confirmed that the death reason for forest musk deer was Yersinia pseudotuberculosis caused by the sepsis, and controlled the disease by the sensitive antibiotic.

关 键 词：假结核耶尔森菌 林麝 16S RDNA 分离鉴定 

分 类 号：S858.94[农业科学—临床兽医学]