大鼠再生肝细胞的分离和培养  

Isolation and Culture of Hepatocytes from Regenerating Liver of Rat

作　　者：杨军英[1,2] 刘芳[1] 李涛[1] 刘慧兵[1] 

机构地区：[1]河南师范大学生命科学学院,省部共建细胞分化调控重点实验室,河南新乡453007 [2]河南师范大学体育学院,河南新乡453007

出　　处：《四川动物》2014年第3期440-444,共5页Sichuan Journal of Zoology

基　　金：河南省基础研究与前沿项目(122300410253)

摘　　要：建立大鼠2/3肝切除模型,分别于术后恢复0 h、6 h、12 h、24 h、48 h、72 h、120 h、168 h等时间点,采用原位胶原酶消化和密度梯度离心法分离、纯化再生肝细胞,获得的再生肝细胞活性95%以上,纯度96%以上。用DMEM培养液添加其他物质后培养再生肝细胞,在培养的96 h内,肝细胞生长状态良好,具有分泌白蛋白和合成尿素的功能。细胞的DNA合成主要在培养24 h以后,72 h时达到高峰。Hepatocytes of rat treated by partial hepatectomy after 0 h, 6 h, 12 h, 24 h, 48 h, 72 h, 120 h, and 168 h were isolated and purified by collagenase digestion and density gradient centrifugation. The viability of regenerated hepatocytes was over 95% , and the purity was more than 96%. The regenerated hepatocytes grow well in DMEM containing 10% fetal calf serum, and were capable of secreting albumin and synthesizing urea. DNA synthesis of hepatoeytes was initiated after 24 h and achieved the peak at 72 h.

关 键 词：大鼠 再生肝细胞 细胞培养 白蛋白 尿素 DNA合成 

分 类 号：S865.12[农业科学—野生动物驯养]