功能化离子液体化学修饰猪胰脂肪酶的催化性能  

Chemical modification of porcine pancreatic lipase by functional ionic liquid

作　　者：初旭明[1] 贾儒[1] 杨姣[1] 黄和[1] 胡燚[1,2] 

机构地区：[1]南京工业大学生物与制药工程学院,南京211800 [2]材料化学工程国家重点实验室,南京210009

出　　处：《生物加工过程》2014年第3期42-46,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家高技术研究发展计划(863计划)(2011AA02A209);国家杰出青年科学基金(21225626);国家自然科学基金青年基金(20906049);国家自然科学基金重点项目(20936002)

摘　　要：选取氯化1-羧甲基-3-甲基咪唑、氯化1-羧甲基-3-乙基咪唑、氯化1-羧甲基-3-丁基咪唑3种离子液体对猪胰脂肪酶(PPL)进行化学修饰,得到3种修饰的脂肪酶分别命名为PPL-M、PPL-E、PPL-B。以三乙酸甘油酯水解为模型反应,考察离子液体修饰前后PPL的活力、热稳定性、耐有机溶剂性等酶学性质,并通过紫外光谱研究修饰对PPL空间结构的影响。结果表明:修饰后PPL的活力明显提高,对温度和pH的敏感度降低。修饰酶的热稳定性明显提高,在高浓度的甲醇及N,N-二甲基甲酰胺(DMF)中仍能保持游离酶活力的100%。修饰后酶的特征吸收峰发生红移,吸收强度增强,修饰后酶的微环境发生了改变。Porcine pancreatic lipase （ PPL ） was modified with functionalized ionic liquids （[HOOCMMIm][Cl],and [HOOCEMIm][Cl],[HOOCBMIm][Cl]）.Activity in buffer and organic solvents,thermostability,and ultraviolet spectrum of the modified PPL were compared with those of the unmodified PPL in the triacetin hydrolysis reaction.The results showed that modified enzymes were more tolerant to temperature and low pH changes and had both higher activity and stability.Functionalized ionic liquids modification enhanced thermal stability of PPL.Enzymes contained 100% activity of PPL in high concentration of methanol and N,N-dimethylformamide（ DMF） after modification.After PPL was modified by functionalized ionic liquids,red shift of PPL characteristic ultraviolet peak and absorbance increase occurred due to changes of lipase microenvironment.

关 键 词：酶 离子液体 化学修饰 猪胰脂肪酶 

分 类 号：TQ423.2[化学工程]