间甲酚对瓯江彩鲤早期发育总ATP酶活性的影响  

Effects of M-cresol on Activity of Total ATPase During the Early Development of Cyprinus carpio var. color

作　　者：朱俊华[1] 梁琍[2] 姚俊杰[1] 冯亚楠[1] 

机构地区：[1]贵州大学动物科学学院水产科学系,贵州贵阳550025 [2]铜仁学院生化系,贵州铜仁554300

出　　处：《水生态学杂志》2014年第3期52-55,共4页Journal of Hydroecology

基　　金：国家自然科学基金(31160527);贵州大学研究生创新基金(研农2013018)

摘　　要：在水温(20±2)℃条件下,设置7 mg/L、13 mg/L、19 mg/L的间甲酚(m-cresol)浓度梯度,研究了瓯江彩鲤(Cyprinus carpio var.color)早期发育过程中总ATP酶活性变化及间甲酚对总ATP酶影响。结果表明,瓯江彩鲤总ATP酶活性在成熟卵和受精卵中无显著差异(P>0.05);各浓度组总ATP酶活性表现为良好的剂量-效应和发育时期-效应关系;总体上看,瓯江彩鲤早期发育从原肠胚期开始,总ATP酶活性随着发育时间的延长和暴露浓度的增加而降低。Activity of total adenosine triphosphatase （ ATPase） was determined during the early development of Cyprinus carpio var.color exposed to 7 mg/L, 13 mg/L and 19 mg/L m-cresol at the temperature of （20 ±2）℃.The influence of m-cresol on the total ATPase was analyzed through biochemical and static toxicological methods .The results showed that the activity of total ATPase had no significant difference in the fertilized eggs and the mature eggs of Cyprinus carpio var.color（ P〉0.05） .However, the activities of total ATPase in all treatment groups dem-onstrated obvious dose-effect relationship and stage-effect relationship in response to m-cresol during the early development of Cyprinus carpio var.color.In general, the activity of total ATPase decreased with the extension of developmental time and increasing of m-cresol concentration from gastrula stage of the early development of Cyprinus carpio var.color.

关 键 词：间甲酚 瓯江彩鲤 早期发育 总ATP酶 

分 类 号：Q556[生物学—生物化学]