土人参提取液对D-半乳糖所致衰老小鼠的抗氧化作用研究  

Antioxidant Effect of Talinum crassifolium Extract on D-Galactose Induced Aging Mice

作　　者：文全泰[1] 潘廷啟[1] 黄礼德[2] 李芸达[1] 赵善民[3] 何显教[3] 黄彦峰[3] 晋玲[3] 

机构地区：[1]右江民族医学院临床学院 [2]百色市人民医院 [3]右江民族医学院生理学教研室,百色533000

出　　处：《天然产物研究与开发》2014年第5期662-665,共4页Natural Product Research and Development

基　　金：右江民族医学院2013年度自治区级大学生创新训练计划立项项目(2012-23)

摘　　要：本文研究了土人参提取液对D-半乳糖所致衰老小鼠的抗氧化作用的影响。取昆明种小白鼠50只,随机分为5组(正常对照组、衰老模型组、土人参提取液低、中、高剂量组)。除正常对照组外,其余各组每天于颈背皮下注射D-半乳糖125 mg/kg,连续42 d,造成亚急性衰老模型。在注射D-半乳糖的同时,三个不同剂量实验组每天分别灌胃土人参提取液5、10和15g/kg,正常对照组及衰老模型组给予等量生理盐水。于42 d后测定小鼠体内超氧化物歧化酶(Super Oxide Dismutase,SOD)、谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-PX)等酶的活性,及丙二醛(Maleic Dialdehyde,MDA)的含量。结果显示:与正常对照组相比,衰老模型组小鼠心脏、肝脏组织SOD及血清中GSH-PX活性降低(P<0.01),MDA含量增加(P<0.01)。与衰老模型组相比,土人参提取液低、中、高剂量组小鼠心脏、肝脏组织SOD及血清中GSH-PX活性明显升高(P<0.01);中、高剂量组小鼠心脏、肝脏组织中MDA含量降低明显(P<0.01),低剂量组小鼠肝脏的MDA含量明显降低(P<0.01)。上述结果表明:土人参提取液对D-半乳糖所致衰老小鼠有抗衰老作用。In the present study,the effect of Talinum crassifolium Extract on antioxidation in aged mice induced by D-galactose was investigated. Fifty male Kunming mice were randomly divided into five groups, namely control group, model group, and T. crassifolium low-, medium- and high-dose groups. Except the control group, all other groups were given with 125 mg/kg of D-galactose by subcutaneous injection at neck nape for 42 d. At the same time,mice in the three sample groups were gavaged with 5,10 and 15 mg/kg of T. crassifolium extract;and mice in control group and model group were gavaged with the same volume of normal saline. The activities of Super Oxide Dismutase （SOD） ,Glutathione perexidase （GSH-PX） ,and the content of Maleie Dialdehyde （MDA） in mice were then determined. The resulted showed that the levels of GSH-PX in blood serum and SOD in liver, heart of the model group were significantly lower than these of normal control group （P 〈 0.01 ）. The activities of MDA in liver and heart of the model group significantly increased compared with these of normal control group （P 〈0.01 ）. The levels of GSH-PX in blood serum and SOD in liver,heart of T. cras- sifolium low-,medium- and high-dose groups were significantly higher than these of model groups （P 〈0.01 ）. The levels of MDA in liver and heart of T. crassifolium medium and high doses groups were significantly lower than these of model groups （P 〈 0.01 ）. The of activity of MDA in liver of T. crassifolium low dose group was significantly lower than that of normal control group （P 〈 0.01 ）. Based on these results, it was concluded that T. crassifolium extract had antiaging effect on D-galactose induced aging mice.

关 键 词：土人参提取液 D-半乳糖 衰老模型 抗氧化作用 

分 类 号：R965[医药卫生—药理学]