HPLC-DAD和UPLC-MS/MS对蔓花生中二苯乙烯类化合物的研究  

Simultaneous Determination of Stilbene Compounds in Arachis duranensis by HPLC-DAD and UPLC-MS /MS

作　　者：韩寒冰[1] 王明阳 马超[1] 李海航[3] 

机构地区：[1]广东石油化工学院化学与生命科学学院 [2]茂名出入境检验检疫局,茂名525000 [3]华南师范大学生命科学学院,广东省植物发育生物工程重点实验室,广州510631

出　　处：《天然产物研究与开发》2014年第5期699-703,712,共6页Natural Product Research and Development

基　　金：广东省科技计划项目(2011B020310008);广东省自然科学基金(S201101000368)

摘　　要：采用HPLC-DAD和UPLC-MS/MS对蔓花生中白藜芦醇、白藜芦醇苷和二苯乙烯苷三种二苯乙烯类化合物进行鉴定,建立HPLC-DAD同时测定三种化合物含量的方法。HPLC-DAD分析采用依利特ODS1色谱柱,柱温40℃,流动相A为甲醇,B为1%乙酸水溶液,流速1.0 mL/min。UPLC-MS/MS采用ACQUITY UPLC BEH C18色谱柱,流动相A为乙腈,B为水,电喷雾离子源,负离子检测,数据采集方式为多反应监测模式(MRM)。结果表明:蔓花生根、茎和叶中白藜芦醇含量分别为66.28、90.83和81.56μg/g,白藜芦醇苷含量分别为123.78,302.77和236.53μg/g,根和茎中二苯乙烯苷含量分别为673.60和764.65μg/g,叶中未检测到二苯乙烯苷。Simultaneous determination of 3 stilbene compounds, namely resveratrol, polydatin and stilbene glucoside （2, 3,5,4＇-tetra-hydroxT-stilbene-2-O-β-D-gluceside） from Arachis duranensis was performed using HPLC-DAD and UPLC- MS/MS. The experimental conditions for I-IPLC-DAD analysis were as follows： ODS1 column （250mm × 4.6 mm 5 μm） ; column temperature, 40℃ ; mobile phase A, methanol; mobile phase B, 1% aqueous acetic acid; flow rate, 1.0 mL/min; DAD scanning wavelength, 190-400 nm; detection wavelength, 303 nm. The UPLC-MS/MS conditions were as follows ：ACQU1TY UPI.,C BEH Cls column （2.1 mmx 50 ram,1.7 pln） ;mobile phase A,acetonitrile;mobile phase B, water;ion source, ESI （negative ion mode）. UPLC-MS/MS data were collected using multiple reaction monitoring （MRM） mode. The results showed that resveratrel contents were 66.28,90.83 and 81.56 μg/g,polydatin contents were 123.78,302.77 and 236.53μg/g, in roots, stems and leaves of A. duranens/s, respectively. The content stilhene glucoside in roots and stems was 673.60 and 764.65 μg/g,respectively,but was not detected in leaves.

关 键 词：蔓花生 二苯乙烯类化合物 HPLC-DAD UPLC-MS MS 

分 类 号：R286[医药卫生—中药学]