银杏内酯B对缺氧缺血性脑损伤新生大鼠脑组织Foxg1基因表达及细胞增殖的影响  

Effect of ginkgolide B on expression of Foxg1 gene and proliferation of cells in brain tissue of newborn rats with hypoxic-ischemic brain damage

作　　者：牛国辉[1] 王军[1] 尚凤伟[1] 朱登纳[1] 张晓莉[2] 

机构地区：[1]郑州大学第三附属医院康复科,450052 [2]郑州大学第三附属医院儿内科,450052

出　　处：《中华实用儿科临床杂志》2014年第10期777-780,共4页Chinese Journal of Applied Clinical Pediatrics

基　　金：河南省教育厅自然科学研究项目 （2009A320039）

摘　　要：目的 观察不同剂量银杏内酯B（GB）对缺氧缺血性腑损伤（HIBD）新生大鼠腑组织Foxg1基因信使核糖核酸（mRNA）表达及细胞增殖的影响.方法 清洁级7日龄SD大鼠128只,按随机数字表法分为假手术组、模型组、GB低剂量组及GB高剂量组,后3组采用经典Rice法制作HIBD动物模型,模型制作4h后GB低剂量组与GB高剂量组分别按5 mg/kg、10 mg/kg腹腔注射GB,其他2组分别注射等量9 g/L盐水,1次/d,共5d.每组随机分别在造模后3d、7d、14 d、28 d处死,荧光定量PCR法检测各时间点Foxg1 mRNA的表达.免疫组织化学技术观察4组大鼠海马齿状回颗粒下层（SGZ）区5-溴脱氧尿核苷阳性细胞的表达,并计数分析.结果 模型组、GB低剂量组及GB高剂量组Foxg1 mRNA的表达均于造模后3 d开始增加,7 d达高峰,7 d后逐渐下降;与假手术组比较,3组在造模后3 d、7 d、14 d、28 d表达均增高;2个治疗组表达又高于模型组,差异均有统计学意义（P均＜0.01）.GB高剂量组在造模后7 d、14 d、28 d表达均高于GB低剂量组,差异均有统计学意义（P均＜0.01）.HIBD后5-溴脱氧尿核苷阳性细胞数增加,GB低剂量组、GB高剂量组均高于模型组,差异均有统计学意义（P均＜0.05）,GB高剂量组阳性细胞数显著高于GB低剂量组,差异有统计学意义（P＜0.05）.结论 HIBD后GB可以上调腑组织的Foxg1 mRNA表达及促进新生细胞增殖;且GB高剂量组较GB低剂量组效果更加明显.Objective To observe the effects of ginkgolide B （GB） on mRNA expression of foxgl and proliferation of cells in brain tissue of newborn rats with hypoxic-ischemic brain damage （HIBD）.Methods A total of 128 clean 7-day-old healthy SD rats were randomly divided into sham operation group,the model group,the low-GB dose and the high-GB dose treatment groups.Classic Rice method were used to establish HIBD models in the latter 3 groups.Four hours after operation,and GB in dose of 5 mg/kg and 10 mg/kg was given to rats in the low and the high dose treatment groups by intraperitoneal injection postoperatively,once a day for 5 days,while sham operation and model groups were treated with equal physiological saline.All groups were respectively sacrificed on 3 d,7 d,14 d,28 d respectively.Quantitative real-time fluorescent polymerase chain reaction was employed to detect expression of Foxg1 gene.Then the number of 5-bromodeoxyuridine positive cell in subgranular zone was investigated by immunolluorescent stairning.Results The Foxg1 mRNA expression was observed 3 days after HIBD,peaked on 7th day,and then declined gradually; the levels of Foxg1 mRNA in the 2 treatment groups were higher than that of the HIBD group （all P 〈 0.01） ; The expression of Foxgl at 7 d,14 d,28 d,in high-dose group were higher than those in the low-dose group （all P 〈 0.01）.The number of 5-bromodeoxyuridine positive cell was increased after HIBD,and the levels in the low-and the high-dose treatment groups were all higher than that of the model group （all P 〈 0.05） ; the number of positive cell in high-dose treatment groups were higher than that in the low-dose treatment groups （P 〈 0.05）.Conclusions GB can promote the expression of Foxg1 gene and improve the proliferation of cells in Brain tissue after HIBD,which shows more significant efficacy in high-dose group than in low-dose group.

关 键 词：缺氧缺血性脑损伤 银杏内酯B Foxg1基因 

分 类 号：R285.5[医药卫生—中药学]