反相高效液相色谱法同时测定血清、尿液中尿酸、肌酐的含量  

Simultaneous determination of creatine and uric acid in urine and serum samples by reversed-phase high performance liquid chromatographic method

作　　者：马晓丽[1] 冉新建[2] 孟磊[1] 李琳琳[2] 毛新民[3] 

机构地区：[1]新疆医科大学分析测试中心,乌鲁木齐830000 [2]新疆医科大学基础医学院,乌鲁木齐830000 [3]新疆医科大学中医学院,乌鲁木齐830000

出　　处：《分析试验室》2014年第6期638-641,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(81260144)资助

摘　　要：建立了同时测定人体血清、尿液中肌酐、尿酸含量的高效液相色谱分析方法。采用Hypersil ODS C18色谱柱,甲醇（A）-20 mmol/L乙酸铵（B）为流动相,梯度洗脱,流速1.0 mL/min,UV检测,检测波长235 nm。血清中肌酐、尿酸含量在2～20μg/mL范围内线性关系良好,尿液中肌酐、尿酸在5～1000μg/mL范围内线性关系良好;平均加标回收率在86.7%～99.0%,RSD小于5%。选择甲醇-乙酸铵作为流动相进行血清/尿液中肌酐、尿酸含量测定具有快速简便的特点,方法应用于健康人和糖尿病病人血清/尿液样本的测定,可以对临床病人进行快速监测。A method for simultaneous determination of uric acid and creatinene in urine and serum samples by high performance liquid chromatography was described. A Hypersil ODS Cls column was used and the mobile phase was 20 mmol/L ammonium acetate buffer with gradient elution, the flow was 1.0 mL/min, and UV detection wavelength was 325nm. The optimum conditions for separation were investigated. Under the optimized conditions, measurements were achieved within 10 min. The liner ranges were 2 - 20 μg/mL in serum samples and 5 ~ 1000 μg/mL in urine samples, respectively. The recoveries were from 86.7% to 99.0% and RSD was less than 5%. In this study, the methanol and ammonium acetate buffer were used to measure the creatinine and uric acid in serum and urine samples, and it was a rapid and simple method ,which can be used to monitor the real diabetic patients and get the real results.

关 键 词：高效液相色谱法 血清 尿液 肌酐 尿酸 

分 类 号：O657.7[理学—分析化学]