HPLC法同时测定24种花中的5种活性成分含量  

Simultaneous determination of five active components in 24 flowers by HPLC

作　　者：彭贵龙[1] 周光明[1] 秦红英[1] 张丽君[1] 

机构地区：[1]西南大学化学化工学院,发光与实时分析教育部重点实验室,重庆400715

出　　处：《分析试验室》2014年第6期646-650,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(21277110)资助

摘　　要：建立了高效液相色谱法（HPLC）同时测定24种花中绿原酸、金丝桃苷、槲皮素、木犀草素和异鼠李素含量的方法。采用C18柱（250 mm×4.6 mm,5μm）,流动相为甲醇-0.2%冰乙酸溶液梯度洗脱,检测波长350 nm,柱温35℃,流速0.8 mL/min。绿原酸、金丝桃苷、槲皮素、木犀草素和异鼠李素分别在0.0208～104.00μg/mL（r=0.99993）,0.017~85.00μg/mL（r=0.99998）,0.0172~86.00μg/mL（r=0.99997）,0.0304~152.00μg/mL（r=0.99997）,0.0168～84.00μg/mL（r=0.99986）范围内与峰面积呈良好的线性关系,平均加标回收率（n=9）92.26%～99.09%,仪器精密度（n=6）RSD均小于3.1%,方法重复性（n=6）的RSD均小于3.6%。方法可同时测定这24种花中绿原酸和黄酮类物质的含量,可作为花中活性成分定量分析的方法。phase was adopted. The UV detective wavelength was 350 nm, and the column temperature was set at 35 ℃. The linear response ranged from 0. 0208 μg/mL to 104. 00 μg/mL for chlorogenic acid （ r = 0. 99993 ）, 0. 017μg/mL to 85.00 μg/mL for quercetin 3-D-galactoside （ r = 0. 99998 ）, 0. 0172 μg/mL to 86. 00μg/mL for quercetin （ r = 0. 99997 ）, 0. 0304 μg/mL to 152.00μg/mL for luteolin （ r = 0. 99997 ）, 0. 0168μg/mL to 84.00μg/mL for isorhamnetin （ r = 0. 99986 ）, respectively （ n = 6 ）. The recoveries （ n = 9 ） of five active components were 92. 26% - 99.09 %. All of RSDs of precision （ n = 6） and repeatability （ n = 6） were less than 3.1% and 3.6%. The contents of chlorogenic acid and flavonoids in the twenty-four flowers were determined by HPLC, and the validation data demonstrated that the method could be used for quantitative determination of the five active components in the flowers.

关 键 词：绿原酸 金丝桃苷 槲皮素 木犀草素 异鼠李素 HPLC 

分 类 号：O657.7[理学—分析化学]