黄芩素与人血清白蛋白相互作用的研究  

Study on the interaction between baicalein and human serum albumin

作　　者：邹婷[1] 周雄威 邹雪晴[1] 张业中[1] 戴捷[1] 

机构地区：[1]长江大学化学与环境工程学院,荆州434023

出　　处：《分析试验室》2014年第6期687-690,共4页Chinese Journal of Analysis Laboratory

基　　金：国家大学生创新性实验计划项目(104892013019)资助

摘　　要：在模拟人体生理条件下,利用荧光光谱法研究了黄芩素(Bcl)与人血清白蛋白(HSA)的相互作用。研究结果表明,黄芩素能够猝灭人血清白蛋白的内源荧光,其猝灭机理为静态猝灭。热力学参数ΔHθ(-70.8 kJ/mol),ΔSθ(-133 J·mol-1·K-1)的值均小于0,表明氢键和范德华力是维持基态复合物稳定性的主要作用力。位点竞争实验表明site I是黄芩素在HSA上的结合部位。三维荧光光谱实验结果表明黄芩素与人血清白蛋白相互作用后,改变了人血清白蛋白的构象。The interaction of baicalein and human serum albumin was investigated via the fluorescence spectroscopy under simulated physiological conditions. The results indicate that the interaction of baicalein and human serum albumin is static process. The values of thermodynamic parameters △H^θ and △S^θdemonstrate that van der Waals interactions and hydrogen bonds play important roles in maintaining the stability of the compound. The site marker competitive experiments show the binding site of Baicalein to HSA locates site 1. The result of three-dimensional fluorescence spectra indicates that baicalein can change the conformation of HSA.

关 键 词：黄芩素 人血清白蛋白 荧光猝灭 三维荧光 

分 类 号：O657.63[理学—分析化学]