我国鸡传染性支气管炎病毒地方分离株S1基因的RFLP基因分型的研究  

The Study on Genotype of S1 Gene of Five Chinese Isolates of Avian Infectious Bronchitis Virus Using RFLP

作　　者：游洪[1] 王林川[1] 

机构地区：[1]华南农业大学动物医学系,广州510642

出　　处：《动物医学进展》2001年第1期47-49,55,共4页Progress In Veterinary Medicine

基　　金：国家自然科学基金重大项目! (398932 90 - 2 B)

摘　　要：采用 RT- PCR方法分离得到了五个IBV地方分离株 ( HN2 ,HN4,JX1 ,SC2和SC4)的 S1基因片段 ,并用限制性内切酶Hae 对五个 IBV地方分离株 S1基因的c DNA进行 RFL P分析。结果显示 ,五个 IBV地方分离株 S1基因 c DNA的 Hae 带型与标准毒株 M41和 Beaudette相同。根据Kwon建立的 RFL P分型标准 ,本试验的五个地方分离株应归属于马萨诸塞血清型。The S1 gene fragment of the five Chinese isolates of IBV(HN2,HN4,JX1,SC2,SC4) had been attained using RT PCR. The S1 gene fragments were digested with restriction enzyme Hae III . The results indicated that the five isolates had the same Hae III RFLP pattern as the M41 and Beaudette strains. Therefore , according to the criterion of Kwon(1993), all these five IBV isolates belong to the Massachusetts serotype.

关 键 词：鸡传染性支气管炎病毒 RT-PCR S1基因 RFLP 分型 

分 类 号：S858.315.3[农业科学—临床兽医学]