补中益气汤对肺腺癌顺铂耐药细胞株移植瘤裸鼠脾、胃和肺中PI3K和AKT表达的影响  

Effect of Buzhong Yiqi decoction on PI3K and AKT in spleen,stomach and lung of nude mice with lung adenocarcinoma transplantation tumor

作　　者：刘亚莉[1,2] 王莹[1] 易佳丽[2] 井欢[1] 刘春英[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110032 [2]辽宁卫生职业技术学院辽宁省基础医学研究所,辽宁沈阳110101

出　　处：《中国中药杂志》2014年第10期1869-1873,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81072743);辽宁省教育厅项目(L2010355)

摘　　要：目的:探讨补中益气汤对肺腺癌顺铂耐药细胞株移植瘤裸鼠脾、胃、肺中PI3K/AKT信号通路的影响。方法:60只裸鼠随机分为:空白对照组、荷瘤对照组、顺铂组、补中益气汤低、中、高剂量组。分别给予相应干预后,测量移植瘤体积,计算抑瘤率。应用免疫组化,Real-time PCR方法检测裸鼠脾、胃、肺组织中PI3K,AKT蛋白和基因的表达水平。结果:不同浓度的补中益气汤联合顺铂能抑制移植瘤的生长,其中以中、高剂量组的抑制作用最强;荷瘤裸鼠的脾、胃、肺中PI3K,AKT蛋白和基因的表达均升高,以荷瘤对照组升高最显著,随着应用顺铂和补中益气汤浓度的增加,PI3K,AKT的表达逐渐降低,在脾、胃组织中与荷瘤对照组比较有统计学差异(P<0.05),补中益气汤中、高剂量组与顺铂组比较有统计学差异(P<0.05),与空白对照组比较无统计学差异。结论:在肺腺癌移植瘤裸鼠中,脾、胃、肺组织中PI3K,AKT的蛋白和基因的表达增加,顺铂和补中益气汤可以降低PI3K,AKT表达,其降低程度与补中益气汤浓度可能存在一定关系;顺铂联合补中益气汤可降低脾、胃、肺中PI3K和AKT蛋白和基因的表达而使该通路接近正常,从而保护脾、胃、肺的功能,PI3K/AKT信号通路上可能有补中益气汤作用的靶点。Objective： To explore the effect of Buzhong Yiqi decoction on PI3K/AKT signaling pathway in spleen,stomach and lung of nude mice with lung adenocarcinoma transplantation tumor.Method： Totally 60 nude mice were randomly divided into the blank control group,the tumor-bearing control group,the cisplatin group,the low-dose Buzhong Yiqi decoction group,the middle-dose Buzhong Yiqi decoction group and the high-dose Buzhong Yiqi decoction group.After the corresponding interventions,efforts were made to measure the transplanted tumor volume and calculate the tumor inhibiting rate.The immunohistochemical method and real time PCR were used to detect the expression of PI3K and AKT level in nude mice spleen,stomach and lung.Result： Buzhong Yiqi decoction of different concentrations combined with cisplatin could inhibit the growth of the transplanted tumor,with the strongest inhibitory effect in the middledose Buzhong Yiqi decoction group and the high-dose Buzhong Yiqi decoction group.All of the expressions of PI3K and AKT protein and gene in the spleen,stomach and lung increased,with the most significant increase in the tumor-bearing group.Along with the increase of the concentration of cisplatin and Buzhong Yiqi decoction,the expressions of PI3K and AKT gradually reduced.Compared with the tumorbearing control group,there were statistical differences in spleen and stomach tissues（P 0.05）.Compared with the cisplatin group,the middle-dose Buzhong Yiqi decoction group and the high-dose Buzhong Yiqi decoction group showed statistical differences（P 0.05）,but without statistical difference compared with the blank control group.Conclusion： Among nude mice with lung adenocarcinoma transplantation tumor,the PI3K and AKT protein and gene expressions in spleen,stomach and lung tissues increased,which might indicated the effect of cisplatin and Buzhong Yiqi decoction in reducing PI3K and AKT expressions and the relations between the reduction degree and the concentrations of Buzhong Yiqi decoction.Cisplatin combined with

关 键 词：补中益气汤 肺腺癌 移植瘤 裸鼠 脾、胃、肺 PI3K AKT 

分 类 号：R273[医药卫生—中西医结合]