空心李组织培养条件研究  

Research on Tissue Culture of Prunus salicina

作　　者：陈卫雪 石开明[1] 李闯[1] 周梦莹[1] 杨秋玲[1] 池政松 

机构地区：[1]湖北民族学院林学园艺学院,湖北恩施445000

出　　处：《园艺与种苗》2014年第2期47-49,53,共4页Horticulture & Seed

基　　金：湖北省教育厅项目(Q20122902);湖北民族学院林学园艺学院大学生创新项目(LXDC1324)

摘　　要：[目的]探索空心李实生苗高效再生系统的培养基类型和培养基配方。[方法]以沿河沙子、香蜜空心、红心道、东门3号4个品种的空心李为试验材料,研究空心李组织培养过程中的一些关键技术措施。以4种李成年嫁接树梢尖、带芽茎段为外植体,研究空心李嫁接树营养器官组织培养的主要调控因素及控制外植体微生物污染的措施,建立完整、高效、实用的空心李组织培养快速繁殖体系。[结果]研究结果表明,春季生长的植物体内存在大量生长素、细胞分裂素和赤霉素等生长调节物质,因而组织培养时成活率高、生长快;不同条件的培养结果不同,空心李种子发苗的最佳方案是成熟种子在半固体1/2MS萌发后,转至固体MS上培养;不同外植体的培养结果是带腋芽的茎段繁殖系数大于茎尖,差异达到显著水平;在激素配比中,细胞分裂素6-BA与生长素NAA浓度配比适当时有利于丛生芽的诱导和分化。[结论]最适合空心李幼穗的6-BA浓度为0.5 mg/L,而健壮梢则为1.0mg/L;NAA浓度以1.0 mg/L为好。The aim was to establish a optimal medium type system, optimum medium for efficient regeneration of Prunus salicina seedlings . [Method]Taking mature embryos of Yanhe plum, Xiangmikongxin plum, Honxindao plum and Dongmen3 plum as materials ,the key technical measures of tissue culture of Prunus salicina were researched. Moreover, according to four grapefruit trees grafted tip and stems with buds as explants, the main regulatory factors of vegetative organs and tissue culture explants and the measures to control microbial contamination were studied. In following that, a complete practical and efficient grapefruit rapid propagation technology system was established. [Result]The results showed that there were a lot of auxin, cytokinins and gibberellins and other growth regulators, and thus tissue culture high survival rate, fast-growing plants growing in spring. What’s more, it presented different results by different culture conditions. The best solution was culturing on MS after the semi-solid 1/2 MS with mature seeds. Culturing results indicated that the axillary buds propagation coefficient greater than the shoot tips of stems and the differences were significant. It also pointed out that 6-BA and NAA concentration ratio was conducive to the induction and differentiation. [Conclusion]This study showed that the most appropriate 6-BA concentration was 0.5mg/L for tender spike, 1.0 mg/L for mature spike, and the NAA concentration was 1.0mg/L as well.

关 键 词：空心李 组织培养 快速繁殖 

分 类 号：S66[农业科学—果树学]