葡萄卷叶伴随病毒ⅢCP基因在E.coli中的表达  

Expression of grapevine leafroll associated Virus 3 coat protein gene in E.coli

作　　者：赵冬兰[1] 朱水芳[2] 张满良[1] 张成良[1] 黄文胜[2] 

机构地区：[1]西北农林科技大学植保学院,陕西杨陵71210 [2]农业部植物检疫实验所,北京100029

出　　处：《西北农业大学学报》2001年第1期88-90,共3页Journal of Northwest Sci-Tech University of Agriculture and Forestry（Natural Science Edition)

基　　金：国家"九五"科技攻关项目! (96 -0 0 4-0 5 -0 8)

摘　　要：将葡萄卷叶伴随病毒 的 CP基因克隆到表达载体 p ET- 30 a中 ,转化大肠杆菌 BL2 1,筛选得到阳性克隆 GB- 1,用 IPTG诱导使其表达。SDS- PAGE电泳分析表明 ,该 CP基因在大肠杆菌 BL2 1中得到大量表达。用该病毒的 CP抗血清通过间接 ELIn this study,the coat protein gene of GLRaV 3 was cloned into expression vector PET 30a,then translated into E.coli BL21,and the recombinant plasmid was obtained.GLRaV 3 coat protein gene was expressed in E.coli through IPTG inducing.And a 35 Ku peculiar protein as expected was showed in SDS PAGE.The antigenicity of this protein using ELISA was verified as well.

关 键 词：葡萄卷叶伴随病毒Ⅲ CP基因 基因表达 基因克隆 

分 类 号：S436.631.1[农业科学—农业昆虫与害虫防治]