基于碳载小尺寸银纳米颗粒的过氧化氢电化学传感器  

Hydrogen Peroxide Biosensor Based on Carbon Supported Ultrafine Silver Nanoparticles

作　　者：王奕琛[1,2] 姜秀娥[1] 

机构地区：[1]中国科学院长春应用化学研究所电分析化学国家重点实验室,长春130022 [2]中国科学院大学,北京100049

出　　处：《分析化学》2014年第5期689-694,共6页Chinese Journal of Analytical Chemistry

基　　金：吉林省自然科学基金(No.201215092);中国科学院院长基金和中国青年基金(No.21105097)资助项目~~

摘　　要：以叶酸为碳源,采用水热法制备碳纳米盘,采用自还原法制备负载在碳纳米盘上的小尺寸银纳米颗粒。通过透射电子显微镜、X射线衍射实验、X射线光电子能谱对其形貌和晶面分布情况进行了表征。同时,将碳载小尺寸银纳米颗粒修饰到玻碳电极表面,构建了过氧化氢（ H2 O2）无酶电化学传感器。电化学循环伏安法（CV）和计时电流法（i-t）的研究结果表明,本传感器对H2O2的还原具有电催化活性,响应时间为1.8 s,对H2O2的检出限为2.2μmol/L,线性范围为0.02~14 mmol/L （R=0.997）。此传感器可以避免抗坏血酸、多巴胺、尿酸、葡萄糖对检测的干扰,长时间使用较为稳定,实际尿样检测结果令人满意。Carbon nanoflakes were prepared by hydrothermal treatment using folic acid as carbon source, and then ultrafine silver nanoparticles were grown on the surface of carbon nanoflakes by self-reduction method. The materials were characterized by transmission electron microscopy （ TEM） , X-ray diffraction （ XRD） , and X-ray photoelectron spectroscopy （ XPS） . The carbon flakes supported Ag nanoparticles were used to construct electrochemical biosensor for the detection of hydrogen peroxide （ H2 O2 ） . The figures of cyclic voltammetry （CV） and chronoamperometry （i-t） revealed that the sensor had prominent electrocatalytic activity for the detection of hydrogen peroxide （ H2 O2 ） with a wide linear range from 0. 02 mmol/L to 14 mmol/L （ R=0. 997） and a rapid response time （1. 8 s） upon the addition of H2 O2 . The detection limit was estimated to be 2. 2 μmol/L. The sensor could avoid interference from ascorbic acid, dopamine, uric acid, glucose, and also showed good stability even during long use. The prepared sensor was successfully used in real samples analysis with satisfying results.

关 键 词：碳载银纳米颗粒 无酶检测 电化学生物传感器 过氧化氢 

分 类 号：O657.1[理学—分析化学] TP212[理学—化学]