茄子绒菌斑病病原菌鉴定及生物学特性研究  

Pathogen identification and biological characteristics of eggplant leaf mold

作　　者：刘志恒[1] 郑川[1] 黄欣阳[2] 唐爽爽[1] 李健冰 焦俊[1] 

机构地区：[1]沈阳农业大学植物保护学院,沈阳110866 [2]辽宁省沈阳市农业技术推广站,沈阳110034

出　　处：《植物保护》2014年第3期58-64,69,共8页Plant Protection

摘　　要：本文针对辽宁省近年茄子设施栽培生产上趋重发生的叶部病害——绒菌斑病,对该病的病原菌进行了鉴定及其生物学特性研究。通过病原菌形态学鉴定,柯赫氏法则证病,并对病菌经rDNA-ITS测序,鉴定该病致病菌为真菌灰毛茄钉孢[Passalora nattrassii(Deighton)U.Braun&Crous]。病菌生物学特性测定结果表明:病菌菌丝生长以PDA+茄叶煎汁培养基为最适;适宜菌丝生长的碳源和氮源分别为可溶性淀粉和胰蛋白胨;适宜温度为25℃;最适pH为6;黑暗条件下菌丝生长较好;菌丝致死温度为59℃,10min。病菌分生孢子萌发试验结果表明,茄叶汁能明显促进其萌发,碳源以麦芽糖、氮源以硫酸铵最适于孢子萌发,孢子萌发的适宜温度为30℃;最适pH为5;光暗交替条件下萌发情况较好;分生孢子致死温度为57℃,10min。The identification and biological characteristics of eggplant leaf mold caused by Passalora nattrassii was studied.Pathogen identification was conducted according to Koch＇s postulates,morphology and molecular method.The results showed that the pathogen of eggplant leaf mold was Passalora nattrassii.The biological characteristics test showed that the optimal temperature for growth was 25 ℃ and the optimal pH was 7.The best medium was PDA added with boiled leaf juice of eggplant.The mycelia grew best in soluble starch as carbon source and in peptone as nitrogen source.The mycelia grew better in darkness,and the lethal temperature was 59 ℃ for 10min.The fresh leaf juice of eggplant could significantly promote the rate of conidia germination.The optimal temperature for conidia germination was 30 ℃ and the optimal pH was 5.The conidia germinated best in maltose as carbon source and in ammonium sulfate as nitrogen source.The conidia germinated better under conditions of alternating light and dark,and the lethal temperature was 57 ℃ for 10min.

关 键 词：茄子绒菌斑病 灰毛茄钉孢 鉴定 rDNA-ITS测序 生物学特性 

分 类 号：S436.411[农业科学—农业昆虫与害虫防治]