参附注射液对缺氧缺血性脑损伤新生大鼠大脑皮质分子伴侣介导的自噬激活的干预作用  

Effects of Shenfu Injection on Chaperone-Mediated Autophagy in Cerebral Cortex of Neonatal Rats with Hypoxic-Ischemic Brain Damage

作　　者：张芳芳[1] 王军[1] 刘芳[1] 刘文强[1] 杨倩倩[1] 

机构地区：[1]徐州医学院附属医院,江苏徐州221002

出　　处：《儿科药学杂志》2014年第6期1-4,共4页Journal of Pediatric Pharmacy

基　　金：江苏省卫生厅科技计划重大项目;编号:H200823

摘　　要：目的:探讨新生大鼠缺氧缺血性脑损伤(HIBD)后大脑皮质分子伴侣介导的自噬(CMA)活性的变化及参附注射液的干预作用。方法:参照Rice法制备HIBD模型。将新生7日龄SD大鼠随机分为假手术组(S组)、生理盐水对照组(C组)、参附注射液治疗组(SF组),每组按照观察时间依次分为6 h、12 h、1 d、3 d、5 d、7 d六个亚组,每个亚组8只大鼠。采用免疫组化法和Western Blot法检测大鼠大脑皮质溶酶体相关膜蛋白-2A(LAMP-2A)的表达情况。结果:免疫组化法和Western Blot法显示LAMP-2A在S组各时间点表达水平差异无统计学意义(P>0.05);C组和SF组LAMP-2A的表达水平在各时间点较S组明显升高(P<0.05),且在3 d达到高峰,随后其表达水平下降;SF组LAMP-2A的表达水平在6 h、12 h、1 d较C组高(P<0.05),但其3 d时峰值表达水平较C组低(P<0.05)。结论:HIBD时存在CMA现象,参附注射液在早期能促进LAMP-2A大量表达,后期则抑制其过度表达,从而对HIBD后的新生大鼠可能具有保护作用。Objective： To investigate the role of chaperone-mediated autophagy （CMA） in cerebral cortex of neonatal rats with hypoxic-ischemic brain damage （HIBD）, and the intervention effects of Shenfu injection. Methods： Models of Sprague Dawley rats on postnatal days 7 with HIBD were established with Rice methods. Sprague-Dawley rats were randomly divided into three groups： sham operation group （ S ）, saline control group （ C ）, and Shenfu injection treatment group （ SF ）. They were further divided into six subgroups （n=8 per subgroup） according to the time points 6 h, 12 h, 1 d, 3 d, 5 d, 7 d. The expression of lysosome-associated membrane protein type 2A （LAMP-2A） in cerebral cortex tissue were determined by western blot and immunohistochemistry method. Results： The expression of LAMP-2A in the group S was the same at the different time points （P〉0.05） ; compared with the group S, the expression of LAMP-2A in the group C and SF was obviously up-regulated at the different time points （P〈O. 05 ）, and reached the peak at 3 d and then gradually declined. Compared with the group C, the expression of LAMP-2A in the group SF was obviously up-regulated at 6 h, 12 h and 1 d （P〈0.05）, but the peak value was decreased （P〈O. 05）. Conclusions： There is a CMA evidence in the resistance of HIBD. Shenfu injection can promote the expression of LAMP-2A at early phage and inhibit the overexpression of it at later period to protect the brain from hypoxic-ischemic brain damage.

关 键 词：新生大鼠 缺氧缺血性脑损伤 CMA 溶酶体相关膜蛋白-2A 参附注射液 

分 类 号：R245[医药卫生—针灸推拿学]