附子中双酯型、单酯型、非酯型生物碱对佐剂性关节炎作用的实验研究  

Study of the Effect of the Diester Alkaloids,Monoester Alkaloids and Non-ester Alkaloids in Fuzi on AA Rats

作　　者：张成龙[1] 尹华[1] 章建华[2] 

机构地区：[1]浙江中医药大学药学院,杭州310053 [2]浙江中医药大学附属第一医院

出　　处：《浙江中医药大学学报》2014年第4期379-382,共4页Journal of Zhejiang Chinese Medical University

基　　金：国家自然科学基金项目(81273772);浙江省自然科学基金项目(Y2101229);浙江省中医药科技计划项目(2010ZA026)~~

摘　　要：[目的]明确附子中双酯型生物碱、单酯型生物碱及非酯型生物碱对佐剂性关节炎大鼠血清一氧化氮(NO)、肿瘤坏死因子(TNF-α)水平及足趾容积变化的影响,初步明确其治疗佐剂性关节炎的药效物质。[方法]雄性wistar大鼠,左后足趾皮内一次性注射弗氏完全佐剂(FCA)0.1mL/只造模,2W后,取造模成功的大鼠,随机分为模型组、单酯型生物碱组、双酯型生物碱组及非酯型生物碱组,各给药组灌胃给予相应生物碱药液,给药2W后,检测血清中NO及TNF-α含量,造模及给药期间同时记录体重及足趾容积变化情况。[结果]与正常组比较,模型组与各给药组大鼠体重增长均受到抑制(P<0.05);与模型组比较,各给药组血清中NO、TNF-α含量亦显著性降低(P<0.01),单酯型组及非酯型组在降低NO作用上较双酯型明显(P<0.05);且各给药组大鼠的足趾容积显著降低(P<0.01),其中单酯型组效果更为明显。[结论]附子中单酯型、双酯型及非酯型生物碱对大鼠佐剂性关节炎有良好的治疗作用,其中单酯型生物碱的治疗作用更为明显。[Objective] Preliminarily clear the effective substance of Fuzi in treatment of AA by comparing the NO, TNF-α and toe volume in AA rats affected by diester alkaloids,monoester alkaloids and non-ester alkaloids. [Methods]Male wistar rats were randomly divided into comparing group and model group. FCA was given in the left rear toe of rat in model group（once a day,0.1mL each）. After 2 weeks ’injection, model rats were randomly divided into model group, diester alkaloids group, monoester alkaloids group and non-ester alkaloids group. Rats in comparing group and model group were given physiological saline and others were given corresponding alkaloids. Detect the NO,TNF-αand toe volume after 2 weeks. [Results]Compared with the model group, the weight gain of rats in other group was suppressed（P〈0.05）. Compared with the model group, the toe volume and the NO, TNF-αof rats in the alkaloids groups reduced significantly（P〈0.01）.The toe volume of rats in monoester alkaloids group reduced more obviously. The NO of rats in diester alkaloids group and non-ester alkaloids group reduced more significantly than the rats in monoester alkaloids group（ P〈0.05）. [ Conclusion] The diester alkaloids, monoester alkaloids and non-ester alkaloids in Fuzi al have a significant effect on AA rats and the effect of the monoester alkaloids is the best.

关 键 词：附子 生物碱 佐剂性关节炎 NO TNF-Α 

分 类 号：R331[医药卫生—人体生理学]