Studies on Genetic Transformation of Fresh-cut Chrysanthemum Using DREB1A Promoted by Stress-induced Promoter rd29A  

作　　者：Haiquan HUANG Qing DUAN Ting JIANG Xinyu DENG Jingjing FEI Jun XU Meijuan HUANG 

机构地区：[1]Faculty of Landscape Architecture,Southwest Forestry University

出　　处：《Agricultural Biotechnology》2014年第2期7-9,共3页农业生物技术（英文版）

基　　金：Supported by Natural Science Foundation of Yunnan Province(2007C213M);Provincial Key Discipline of Landscape Plant&Ornamental Horticulture of Yunnan Province;Provincial Key Lab of Colleges and Universities in Landscape Plants and Ornamental Horticulture of Yunnan Province;Large Apparatuses Sharing Platform of Southwest Forestry University

摘　　要：In this study, DERB1A transcription factor and stress-induced promoter rd29A were isolated respectively and amplified from Arabidopsis thaliana, se- quenced and analyzed by DNAsis. In addition, the stress-induced promoter rd29A was utilized to construct the plant expression vector of DERB1A, which was transformed into Agrobacterium tumefaciens. Furthermore, the transgenic regeneration system of fresh-cut chrysanthemum from callus to plantlets was established successfully. On this basis, chrysanthemum leaf-disc explants were genetically transformed with Agrobacterium-mediated method. Two positive transgenie plantlets were obtained in vitro. Based on PCR detection, DREB1A transcription factor was integrated into chrysanthemum genome, which laid the foundation for breeding new transgenie cultivars of fresh-cut chrysanthemum with high comprehensive stress resistance, good cmalitv and high field.In this study, DERB1A transcription factor and stress-induced promoter rd29A were isolated respectively and amplified from Arabidopsis thaliana, se- quenced and analyzed by DNAsis. In addition, the stress-induced promoter rd29A was utilized to construct the plant expression vector of DERB1A, which was transformed into Agrobacterium tumefaciens. Furthermore, the transgenic regeneration system of fresh-cut chrysanthemum from callus to plantlets was established successfully. On this basis, chrysanthemum leaf-disc explants were genetically transformed with Agrobacterium-mediated method. Two positive transgenie plantlets were obtained in vitro. Based on PCR detection, DREB1A transcription factor was integrated into chrysanthemum genome, which laid the foundation for breeding new transgenie cultivars of fresh-cut chrysanthemum with high comprehensive stress resistance, good cmalitv and high field.

关 键 词：DREB1A transcription factor Stress-induced promoter rd29A Fresh-cut chrysanthemum Genetic transformation 

分 类 号：S682.11[农业科学—观赏园艺]