五子衍宗方对环磷酰胺致小鼠DNA损伤的影响  

Effect of Wuzi Yanzong Fang Against Cyclophosphamide Induced DNA Damage in Mice

作　　者：刘苗苗[1,2] 袁丁[1] 黄威峰 彭奔 张长城[1] 

机构地区：[1]三峡大学医学院,湖北宜昌443002 [2]三峡大学化学与生命科学学院,湖北宜昌443002

出　　处：《中国中医药信息杂志》2014年第6期38-40,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81173375)

摘　　要：目的观察五子衍宗方对环磷酰胺(CTX)致小鼠DNA损伤的影响,并探讨其作用机制。方法将BalB/c小鼠随机分为正常组、模型组、五子衍宗方低剂量组、五子衍宗方高剂量组。五子衍宗方低、高剂量组预给药1周后,除正常组外,其余各组腹腔注射CTX 100 mg/kg造模,隔日1次,共3次,同时五子衍宗方低、高剂量组继续给药,末次给予CTX后12 h处死小鼠,分离血清,ELISA检测血清中8-羟基脱氧鸟苷(8-OHdG)的含量,应用单细胞凝胶电泳技术检测骨髓细胞DNA损伤。结果五子衍宗方低、高剂量组能够降低血清中8-OHdG的含量,使小鼠骨髓细胞彗星图像的Olive尾矩、尾距、尾长和尾部DNA含量降低。结论五子衍宗方对CTX致小鼠DNA损伤具有较好的保护作用。Objective To study protective effects of Wuzi Yanzong Fang on DNA damage induced by cyclophosphamide （CTX） in mice, and explore its mechanism. Methods BalB/c mice were randomly divided into normal group, model group, Wuzi Yanzong Fang low dose group and Wuzi Yanzong Fang high dose group. Mice in Wuzi Yanzong Fang groups were pretreated with Wuzi Yanzong Fang for 7 days, then the mice in Wuzi Yanzong Fang groups and model group were intraperitoneally injected with CTX （100 mg/kg） every other day for three times, and mice in Wuzi Yanzong Fang groups were continued administered with Wuzi Yanzong Fang. Animals were sacrificed in twelve hours after the final treatment of CTX. ELISA was used to detect 8-OHdG content in serum, and single cell gel electrophoresis to detect DNA damage in bone marrow cells. Results Wuzi Yanzong Fang low dose group and high dose group reduced the level of 8-OHdG in serum. Wuzi Yanzong Fang significantly decreased Olive tail moment, tail moment, tail length and tail DNA%in mouse bone marrow cells. Conclusion Wuzi Yanzong Fang has good protective effects on DNA damage caused by CTX.

关 键 词：五子衍宗方 环磷酰胺 DNA损伤 小鼠 

分 类 号：R285.5[医药卫生—中药学]