HPLC同时测定尿塞通胶囊中丹参素钠、橙皮苷及丹酚酸B含量  

HPLC Simultaneous Determination of Sodium Danshensu, Hesperidin and Salvianolic Acid B in Niaosaitong Capsules

作　　者：王绍青[1] 金星 张清波[1,2] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]黑龙江省食品药品检验检测所,黑龙江哈尔滨150001

出　　处：《中国中医药信息杂志》2014年第6期83-85,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：2011年国家药品标准提高行动计划(595)

摘　　要：目的：建立同时测定尿塞通胶囊中丹参素钠、橙皮苷、丹酚酸B含量的方法。方法采用高效液相色谱法,C18色谱柱,以乙腈-0.1%磷酸为流动相进行梯度洗脱,流速1.0 mL/min,柱温30℃,检测波长286 nm。结果丹参素钠、橙皮苷、丹酚酸 B 分别在0.03844～1.922μg、0.0002070528～4.044μg、0.03428～1.714μg范围内线性关系良好,平均回收率为90.9%～107.1%,RSD为2.1%～3.2%。结论所建立的方法简便、准确,可用于尿塞通胶囊的质量控制。Objectives To establish an HPLC method for determination of sodium Danshensu, hesperidin and salvianolic acid B in Niaosaitong capsules. Methods Three components were separated by C18 chromatographic column with a gradient mobile phase consisting of acetonitrile and 0.1%phosphoric acid solution at a flow rate of 1.0 mL/min, column temperature of 30 ℃, and detection wavelength was set at 286 nm. Results Sodium Danshensu, hesperidin and salvianolic acid B had good linearity in the range of 0.038 44-1.922 μg, 0.000 207 052 8-4.044 μg, 0.034 28-1.714 μg respectively, and the average recovery rate was 90.9%-107.1%, RSD was 2.1%-3.2%. Conclusion The methods is simple and accurate, and can be used for the quality control of Niaosaitong capsules.

关 键 词：尿塞通胶囊 丹参素钠 橙皮苷 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]