稳定同位素氘标记苏丹红I的同位素丰度和化学纯度分析  

Isotopic Abundance and Chemical Purity Analysis of Stable Isotope Deuterium Labeled Sudan I

作　　者：蔡银萍 雷雯[1] 郑波[1] 杜晓宁[1] 

机构地区：[1]上海化工研究院,上海200062

出　　处：《同位素》2014年第2期65-71,共7页Journal of Isotopes

基　　金：国家科技支撑计划课题(2012BAK25B03-15)

摘　　要：为建立高灵敏度、高选择性的食品中非法添加物的分析检测方法,采用"质量簇"计算分类方法,利用液质联用仪检测稳定同位素氘标记苏丹红Ⅰ的同位素丰度,用于同位素稀释质谱法的内标试剂。针对不同操作人员、不同仪器进行重复性和再现性实验,RSD均<0.1%,重复性和再现性良好。对结果进行了F检验和t检验,结果表明,苏丹红I-D_5的同位索丰度分析方法的准确度和精密度良好。利用高效液相色谱仪,采用外标法,建立标准曲线,确立稳定同位素氘标记苏丹红I-D_5的化学纯度分析方法。结果表明,制备的稳定同位素氘标记苏丹红I-D_5可作为同位素稀释质谱法的内标物质。It is important that to analysis of the isotopic abundance and chemical purity of Sudan I-D5, which is the internal standard of isotope dilution mass spectrometry. The isotopic abundance of Sudan I-D5 is detected by ＂mass cluster＂ classification method and LC- MS. The repeatability and reproducibility experiments were carried out by using different mass spectrometers and different operators. The RSD was less than 0.1%, so the repeatability and reproducibility were satisfactory. The accuracy and precision of the isotopic a bundance analysis method was good with the results of F-test and t-test. The high perform ance liquid chromatography （HPLC） had been used for detecting the chemical purity of Su- dan I-D5as external standard method.

关 键 词：氘标记 苏丹红Ⅰ 液质联用仪 同位素丰度 高效液相色谱法 

分 类 号：O628.2[理学—有机化学]