建立健康者血清胃蛋白酶原参考值区间的初步研究  

Preliminary research on the establishment of reference interval of serum pepsinogen in healthy people

作　　者：陈虹亮[1] 滕文友 袁红霞[1] 朱乐攀[1] 张扬南[1] 黄常洪[1] 刘伟[1] 代国知[1] 

机构地区：[1]郴州市第一人民医院检验医学中心,湖南郴州423000

出　　处：《国际检验医学杂志》2014年第10期1251-1252,1255,共3页International Journal of Laboratory Medicine

基　　金：郴州第一人民医院课题资助(N2012-005)

摘　　要：目的探讨健康者血清胃蛋白酶原（PG）的分布特征并建立其参考值范围。方法选择健康体检者3 753例,根据年龄将其分为〈45岁、45~〈60岁、≥60岁组。采用双抗体夹心酶联免疫吸附测定法进行PGⅠ、Ⅱ检测。结果不同年龄段男、女性健康者血清PGⅠ、PGⅡ及PGⅠ/PGⅡ检测结果均呈偏态分布（P〈0.05）。女性健康者血清PGⅠ、PGⅠ/PGⅡ水平显著高于男性（P〈0.01）。相同年龄,男、女性健康者血清PGⅡ水平的差异无统计学意义（P〉0.05）。相同性别,不同年龄组间PGⅠ水平两两比较,差异无统计学意义（P〉0.05）;PGⅡ水平随着年龄增大而升高（P〈0.01）;PGⅠ/PGⅡ水平随着年龄增大而减低（P〈0.05）。采用百分位数法确定其95%参考值范围,PGⅠ取双侧参考值区间（P2.5~P97.5）,PGⅡ取单侧上限（≤P95）,PGⅠ/PGⅡ取单侧下限（≥P5）。结论健康人群血清PGⅠ、PGⅡ、PGⅠ/PGⅡ参考值区间的制定可以为胃病的防治工作提供依据。Objective To investigate the distribution characteristics of serum pepsinogen （PG） in healthy people and its reference interval establishment .Methods 3 753 healthy people were enrolled and divided into ＆lt;45-year old ,45- ＆lt;60-year old and ≥60-year old group according to their ages .Double antibody sandwich enzyme-linked immunosorbent assay was used to detect PG Ⅰ ,Ⅱ . Results Detection results of serum PG Ⅰ ,PGⅡ and PGⅠ /PGⅡ of male and female healthy people in different age group showed a skewed distribution（P〈0 .05） .Serum PGⅠ and PGⅠ /PGⅡlevels of females were significantly higher than males（P〈0 .01） .In the same age group ,difference of serum PGⅡ levels between males and females was not statistically significant （P〉0 .05） .In the same gender ,pairwise comparison of PGⅠlevels was conducted in different age groups ,and the difference showed no statistical sig-nificance（P〉0 .05） .PGⅡlevel increased with age increasing （P〈0 .01） while PGⅠ /PGⅡlevel increased with age reducing （P〈0 .05） .Percentile method was adopted to determine the 95% reference interval ,the bilateral reference intervals （P2 .5 - P97 .5 ） was taken for PGⅠ ,unilateral upper limit（≤ P95 ） for PGⅡ and unilateral limit （≥ P5 ） for PGⅠ /PGⅡ .Conclusion The establishment of serum PG Ⅰ ,PG Ⅱ ,PG Ⅰ /PG Ⅱ reference intervals of healthy people provides a basis for the prevention and treatment for stomach disease .

关 键 词：胃蛋白酶原类 参考值 酶联免疫吸附测定 健康者 

分 类 号：R446.6[医药卫生—诊断学]