药疹患者外周血淋巴细胞亚群的检测及其意义  

Detection of lymphocyte subsets in peripheral blood of patients with drug eruption and its significance

作　　者：谭飞[1,2] 莫小辉 陈佳 章楚光 胡婷婷 吴飞 宋宁静 顾军[1] 

机构地区：[1]第二军医大学附属长海医院皮肤科,上海200433 [2]上海市皮肤病医院中心实验室,上海200443

出　　处：《国际检验医学杂志》2014年第10期1266-1267,1269,共3页International Journal of Laboratory Medicine

基　　金：上海市公共卫生重点学科建设项目(12GWZX0902)

摘　　要：目的分析药疹患者外周血淋巴细胞亚群的变化。方法将18例初诊药疹患者作为药疹组,根据致敏药物的不同将其分为头孢菌素组(n=9)、青霉素组(n=5)及中药组(n=4),将20例健康体检者作为对照组。应用流式细胞仪检测其外周血T淋巴细胞(CD3+、CD3+CD4+、CD3+CD8+)、B淋巴细胞、自然杀伤细胞(NK)和自然杀伤T淋巴细胞(NKT)所占百分比及其绝对计数。结果药疹组患者外周血T淋巴细胞(CD3+、CD3+CD4+)、B淋巴细胞和NKT细胞所占百分比与对照组的差异有统计学意义(P<0.05);药疹组患者CD3+CD8+淋巴细胞所占百分比与对照组的差异无统计学意义(P>0.05),而其T、B淋巴细胞亚群的绝对计数与对照组的差异有统计学意义(P<0.05)。结论药疹患者外周血CD3+、CD3+CD4+淋巴细胞所占百分比降低,NKT细胞所占百分比增高,这可能与患者免疫调节有关。Objective To analyze the changes of lymphocyte subsets in peripheral blood of patients with drug eruption . Methods 18 newly diagnosed patients were served as the drug eruption group ,and were subdivided into cephalosporin group （n=9） ,penicillin group（n=5） and Chinese medicine group（n=4） according to different sensitizing drugs .20 healthy people were taken as the control group .Flow cytometry were utilized to detect the percentages and absolute counts of T lymphocytes （CD3^＋ ,CD3^＋CD4^＋ and CD3^＋CD8^＋ ） ,B lymphocytes ,natural killer cell（NK） and natural killer T lymphocytes（NKT） in their peripheral blood . Results Differences of percentages of T lymphocytes （CD3^＋ ,CD3^＋ CD4^＋ ） ,B lymphocytes ,NKT cells between the drug eruption group and the control group showed statistical significant （P〈0 .05） .Difference of percentages of CD3^＋ CD8^＋ lymphocytes of pa-tients between the drug eruption group and the control group demonstrated no statistical significant （P＆gt;0 .05） ,while that of abso-lute counts of T and B lymphocytes of patients was statistical significant between the drug eruption group and the control group （P〈0 .05） .Conclusion The percentages of CD3^＋ ,CD3^＋CD4^＋ lymphocytes of patients with drug eruption decrease ,while those of NKT cells increase ,which may be related to the patients′immune regulation .

关 键 词：药疹 淋巴细胞亚群 流式细胞术 外周血 

分 类 号：R593[医药卫生—内科学]