罕见豚鼠耳炎奴卡菌的鉴定及其药物敏感性分析  

Identification of rare Nocardia otitidiscaviarumand its drug sensitivity analysis

作　　者：张薇[1] 彭国钧[1] 刘佳[1] 胡红焱[1] 梁慧[1] 

机构地区：[1]武警总医院检验科,北京100039

出　　处：《国际检验医学杂志》2014年第10期1320-1321,1325,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨罕见豚鼠耳炎奴卡菌的鉴定方法并评价其药物敏感性。方法采用形态、生理生化表型鉴定与16S rRNA序列分析相结合的方法鉴定菌株,使用微量肉汤法检测其药物敏感性。结果临床分离的奴卡菌被鉴定为豚鼠耳炎奴卡菌,其对哌拉西林、红霉素、头孢他啶、头孢哌酮、庆大霉素、头孢西丁、哌拉西林/他唑巴坦耐药,万古霉素、左氧氟沙星、亚胺培南、依替沙星、环丙沙星、阿米卡星、复方磺胺甲基异噁唑敏感。结论 16SrRNA基因序列分析法可用以鉴定豚鼠耳炎奴卡菌,磺胺类药物治疗有效。Objective To study the identification of rare Nocardiaotitidiscaviarum and its drug sensitivity .Methods Morpho-logical ,physiological and biochemical phenotype identification methods combined with 16S rRNA sequence analysis were employed to identified the bacteria strains .Broth method was used to detect their drug sensitivity .Results The clinically isolated Nocardia strain was identified as Nocardiaotitidiscaviarum which was resistant to piperacillin ,erythromycin ,ceftazidime ,cefoperazone ,gen-tamicin ,cefoxitin ,piperacillin/tazobactam ,and was sensitive to vancomycin ,levofloxacin ,imipenem ,etimicin ,ciprofloxacin ,amika-cin ,sulfamethoxazole .Conclusion 16S rRNA gene sequence analysis can be used to identify Nocardia otitidiscaviarum ,and sulfa drugs shows good therapeutic effect .

关 键 词：16SrRNA 序列分析 豚鼠耳炎奴卡菌 

分 类 号：R446.5[医药卫生—诊断学]