Sysmex CA-1500全自动血凝仪PT-der法和Von-Clauss法检测纤维蛋白原的分析  

Analysis on fibrinogen detection by using PT-der assay and Von-Clauss assay on Sysmex CA-1500 Automated Coagulation Analyzer

作　　者：刘振杰[1] 何燕香[1] 徐宁[1] 何文军[1] 吴子安[1] 黄丽英[1] 李涛[1] 

机构地区：[1]广东省中医院检验科,广东广州510370

出　　处：《国际检验医学杂志》2014年第10期1333-1334,1370,共3页International Journal of Laboratory Medicine

摘　　要：目的评价Sysmex CA-1500全自动血凝仪PT-der法和Von-Clauss法测定血浆纤维蛋白原（Fib）的相关性和准确性。方法采用Sysmex CA-1500全自动血凝仪PT-der法和Von-Clauss法分别测定755例血液标本的血浆Fib浓度;高Fib浓度标本的稀释比例为1∶8、2∶7、3∶6、4∶5、5∶4、6∶3,以稀释比例为横坐标,以2种方法测得的Fib浓度为纵坐标,进行简单线性回归分析。结果 Fib：2.0~〈6.0g/L时,Von-Clauss法检测Fib值高于PT-der法（P〈0.01）;Fib：〈2.0g/L,〉6.0g/L时,PT-der法检测Fib值高于Von-Clauss法（P〈0.01）。PT-der法和Von-Clauss法检测的线性回归方程分别为：Y=4.537 7 X＋1.551 3（R2=0.897 3）,Y=7.792 2 X＋0.290 0（R2=0.980 5）。结论 Von-Clauss法能较好地反映人体内具有凝血功能的Fib水平。Objective To evaluate the relevance and accuracy of fibrinogen （Fib） detection by using PT-der assay and Von-Clauss assay on Sysmex CA-1500 Automated Coagulation Analyzer .Methods PT-der assay and Von-Clauss assay on Sysmex CA-1500 Automated Coagulation Analyzer were employed to detect the plasma Fib concentrations of 755 blood samples .The dilution ratios of samples with high Fib concentration were 1∶8 ,2∶7 ,3∶6 ,4∶5 ,5∶4 ,6∶3 ,respectively .The dilution ratios were served as the abscissa ,and the Fib concentrations measured by two methods as the ordinate ,a simple linear regression analysis was per-formed .Results When Fib concentration was in 2 .0- ＆lt;6 .0 g/L ,the Fib value obtained by Von-Clauss assay was higher than that by PT-der assay（P〈0 .01） .When Fib concentration was below 2 .0 g/L or above 6 .0 g/L ,the Fib value obtained by PT-der assay was higher than that by Von-Clauss assay（P〈0 .01） .The linear regression equations of PT-der assay and Von-Clauss assay were Y=4 .537 7X＋1 .551 3（R2 =0 .897 3） ,Y = 7 .792 2X＋ 0 .290 0（R2 =0 .980 5） ,respectively .Conclusion Von-Clauss assay can better reflect the Fib level of human body which has a blood clotting function .

关 键 词：纤维蛋白原 Von-Clauss法 PT-DER法 线性回归 

分 类 号：R446.6[医药卫生—诊断学]