高效毛细管电泳同时测定川西獐芽菜中龙胆苦苷和獐芽菜苦苷  

Simultaneous Determination of Gentiopicroside and Swertiamarin in Swertia mussotii Franch by HPCE

作　　者：高瑞斌[1] 董树清[2] 杨艳[3] 倪京满[3] 赵亮[2] 郭玫[1] 

机构地区：[1]甘肃中医学院药学系,甘肃省高校中(藏)药化学与质量研究省级重点实验室,甘肃兰州730000 [2]中国科学院兰州化学物理研究所,中国科学院西北特色植物资源化学重点实验室,甘肃省天然药物重点实验室,甘肃兰州730000 [3]兰州大学药学院,甘肃兰州730000

出　　处：《辽宁中医杂志》2014年第5期994-997,共4页Liaoning Journal of Traditional Chinese Medicine

摘　　要：目的：建立高效毛细管电泳（CE）同时测定川西獐芽菜中龙胆苦苷及獐芽菜苦苷的方法。方法：研究运行缓冲液的浓度、pH、添加剂β-环糊精浓度、检测波长及分离电压对分离结果的影响。在最佳分离测定条件下,龙胆苦苷和獐芽菜苦苷得到快速分离检测。结果：龙胆苦苷和獐芽菜苦苷分别在9.375-150μg/mL、6.252-100μg/mL范围内线性关系良好,r值分别为0.9997和0.9998,加样回收率分别为99.74%和99.58%,保留时间RSD值分别为6.7%和5.1%,峰面积的RSD值分别为1.98%和2.43%。结论：本方法简便、快速、重现性好,适用于含有龙胆苦苷、獐芽菜苦苷类药物成分的检测。Objective ：The highly performance capillary electrophoresis （HPCE） method was developed for the simultaneous determination of Gentiopicroside and Swertiamarine in Swertia mussotii Franch. Methods ： The effects of some important factors such as pH value, concentration of running buffer, separation voltage and detection wavelength, were investigated. Under the optimal electrophoresis conditions,gentiopicroside and swertiamarine can be well separated and detected fast. Results：The linear range of Gentiopicroside and Swertiamarine were 9. 375 - 150 μg/mL,6. 252 - 100 μg/mL with correlation coefficients of 0. 9997 and 0. 9998. The average recoveries of gentiopieroside and swertiamarine in sample were ranged from 99.74% and 99.58% , the RSD values of retention times were 6.7% and 5.1% ,and those of peak areas were 1.98% and 2.43%. Conclusion：The method was simple, convenient, rapid, reproducibility, and can be successfully applied in the determination of Gentiopicroside and Swertiamafine as pharmaceutical ingredients.

关 键 词：川西獐芽菜 高效毛细管电泳法 龙胆苦苷 獐芽菜苦苷 

分 类 号：R927.2[医药卫生—药学]