穿山龙总皂苷对CIA大鼠关节滑膜组织AP-1表达的影响  

Effects of total saponin from Rhizoma Dioscoreae Nipponicae on the expression of AP-1 in synovium of CIA rats

作　　者：高亚贤[1] 王永为[1] 肖丽君[1] 郭亚春[1] 宋鸿儒[1] 高雅佳[1] 

机构地区：[1]承德医学院,河北承德067000

出　　处：《时珍国医国药》2014年第5期1043-1045,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30873420)

摘　　要：目的观察穿山龙总皂苷对胶原诱导性关节炎(CIA)大鼠关节滑膜组织激活蛋白-1(activator protein-1,AP-1)mRNA表达水平及活性的影响,旨在探讨穿山龙总皂苷治疗类风湿性关节炎的作用机制。方法将造模成功的36只CIA大鼠随机分为CIA模型组、雷公藤组(阳性对照组)、穿山龙总皂苷组,另设正常对照组,每组12只。造模成功以后分别给予相应的药物灌胃,连续治疗35 d。应用原位杂交方法检测滑膜组织中AP-1的两个亚单位c-fos和c-jun的mRNA表达水平;凝胶电泳迁移率实验检测滑膜组织核蛋白提取物AP-1的DNA结合活性。结果与正常对照组相比,CIA模型组大鼠滑膜组织中c-fos和c-jun的mRNA表达水平以及滑膜细胞核蛋白提取物中AP-1的DNA结合活性均显著增高(P<0.01);与CIA模型组相比,穿山龙总皂苷组、雷公藤组的c-fos和c-jun的mRNA表达水平以及滑膜细胞核蛋白提取物中AP-1的DNA结合活性均显著降低(P<0.01)。结论穿山龙总皂苷可能通过抑制转录因子AP-1来调控血管新生相关基因的表达,抑制血管新生来起到治疗RA的作用。Objective To observe the effects of total saponin from Rhizoma Dioscroeae Nipponicae （RDN） on expression of acti- vator protein -1 （AP- 1 ） mRNA and activity in synovium of collagen- induced arthritis （CIA） rats,and investigate the underly- ing mechanism about signal transduction pathways of RDN on antiangiogenesis in treating rheumatoid arthritis. Methods Twelve rats were selected randomly from fifty -two Wistar rats as normal control group, the remaining forty rats were established CIA rat model. Thirty - six successful models of CIA rats were randomly divided into 3 groups （n = 12/group） ：CIA model group,Triptery- gium （TP） - treating group （positive control group）, RDN- treating group, then the rats of treated groups were lavaged with cor- responding medicine for 35 days. In situ hybridization was used to detect the expressions of c - fos mRNA and c - jun mRNA in synovium;The AP - 1 DNA - binding activity in synovium was detected by electrophoretic mobility shift assay （EMSA）. Re- sults Compared to normal control group, c - fos and c - jun mRNA expressions in synovium and the AP - 1 DNA - binding activity in nuclear extract of CIA model group rats＇synovium obviously increased（P 〈 0.01 ）. Compared to CIA model group, c -fos and c - jun mRNA expressions in synovium and the AP - 1 DNA - binding activity in nuclear extract of rats in Tripterygium treatment group and total saponin from RDN treatment group were remarkably decreased （P 〈 0.01 ）. Conclusion Considering that the total saponin from RDN could regulate the expression of angiogenesis related genes maybe related to inhibiting the activator protein - 1 signal transduction pathway, thereby to restrain angiogenesis of rheumatoid arthritis.

关 键 词：胶原诱导性关节炎 穿山龙总皂苷 血管新生 AP-1 

分 类 号：R285.5[医药卫生—中药学]