土鳖虫活性组分对过氧化氢损伤血管内皮细胞的保护作用  

Protective Effects of Eupolyphaga Constituent on Human Umbilical Vein Endothelial Cells Injury Induced by H_2O_2

作　　者：杜清华[1] 曹唯仪[1] 王宏涛 赵韶华 王玉蓉[1] 

机构地区：[1]北京中医药大学,北京100102 [2]河北以岭医药研究院,河北石家庄050035

出　　处：《中医药信息》2014年第3期10-14,共5页Information on Traditional Chinese Medicine

基　　金：国家973重点基础研究计划(No.2012CB518606)

摘　　要：目的：考察土鳖虫抗凝活性组分对过氧化氢损伤的人脐静脉血管内皮细胞（HUVEC）的保护作用。方法：通过细胞存活率（MTT）法确定给药剂量;建立HUVEC细胞损伤模型,测定不同培养时间下细胞培养液中乳酸脱氢酶（LDH）漏出率,以及超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽过氧化物酶（GSH-Px）、一氧化氮（NO）含量。结果：与空白组比较,H2O2（1.1mmol/L）损伤2h可导致细胞存活率降低,模型组SOD、GSH-Px含量显著降低,细胞分泌NO水平降低,LDH漏出率升高,生成MDA增多。与模型组比较,土鳖虫抗凝活性组分在250~4000mg/L浓度范围内能使LDH漏出率降低,显著升高SOD、GSH-Px含量,抑制MDA生成。结论：土鳖虫抗凝活性组分可在一定程度保护血管内皮细胞,有后续研究价值。Objective： To study the protective effects of eupolyphaga anticoagulant constituent（ EAC） on human umbilical vein endothelial cells induced by H2O2. Methods： The MTT assay was used to determinate the suitable dose of eupolyphaga anticoagulant constituent. HUVECs injury model was established after dosing,and the leakage rate of lactate dehydrogenase（ LDH）,superoxide dismutase（ SOD）,malonal dehyde（ MDA）,glutathione peroxidase（ GSH-Px） and nitric oxide（ NO） content of endothelial cell medium of HUVECs were measured to evaluate the protective effect of EAC. Results： Compared with the control group,H2O2（ 1. 1mmol / L） could decrease HUVECs survival rate,which indicated successful modeling; the leakage rate of LDH and the content of MDA increased,while SOD,GSH-Px,and NO decreased significantly in the model group. However,the EAC,of which concentrations range from 250 to 4000mg / L could significantly reduce LDH release and the content of MDA,increase the activity of SOD and GSH-Px compared with the model group. Conclusion： EAC is proved to be worth further study because of its protective effects on HUVEC.

关 键 词：土鳖虫抗凝活性组分 血管内皮细胞 过氧化氢 

分 类 号：R285.5[医药卫生—中药学]