金雀异黄素对氧化损伤的人晶状体上皮细胞凋亡的防护作用  

Genistein Protecting Against Apoptosis of Human Lens Epithelial Cell Injured by Oxidation

作　　者：林瑶[1] 黄秀榕[1] 祁明信[2] 黄露芬[1] 郭娜[2] 唐宋文[1] 陈旭征[3] 

机构地区：[1]福建中医药大学中西医结合学院,福建福州350108 [2]福建中医药大学附属第二人民医院眼科,福建福州350003 [3]福建中医药大学中西医结合研究院,福建福州350108

出　　处：《江西中医学院学报》2014年第1期67-70,共4页Journal of Jiangxi College of Traditional Chinese Medicine

基　　金：福建省教育厅基金(JA12177)

摘　　要：目的:探讨金雀异黄素(Gen)对氧化损伤的人晶状体上皮细胞(HLEC)凋亡有无抑制作用。方法:本研究在终浓度3×10-4mol/L H2O2培养液中复制HLEC凋亡模型,并以雌二醇(E2)作为阳性对照,采用流式细胞仪(FCM)检测HLEC凋亡率和线粒体膜电位(ΔΨM)的变化。结果:FCM结果显示:H2O2组HLEC凋亡率显著高于空白对照组;E2和Gen组凋亡率均低于H2O2组(P<0.01)。H2O2组线粒体膜电位比对照组显著下降;E2组和Gen组的线粒体膜电位均比H2O2组升高(P<0.01)。结论:Gen可减轻实验性氧化损伤的HLEC凋亡程度。Objective： To investigate if Genistein（ Gen） inhibits the apoptosis of human lens epithelial cell（ HLEC） resulted from the experimental oxidative injury. Methods： HLECs were incubated with 3 × 10^- 4mol / L H2O2. And make estradiol（ E2） as a positive control. HLECs apoptosis and apoptosis rate were measured by FCM. Results： Apoptosis rate in H2O2group was significandy higher than that in control group,E2and Gen group. The mitochondria transmembrane potential in H2O2group reduced obviously,comparing to control group,E2and Gen group. Conclusion： Gen may decrease the apoptosis of HLEC resulted from the experimental oxidative injury,providing scientific evidence for pursue effective medicine to prevent and treat cataract.

关 键 词：金雀异黄素 氧化损伤 晶状体上皮细胞 凋亡 

分 类 号：R285.5[医药卫生—中药学]