1800 MHz模拟手机电磁场长期辐射对NIH/3T3细胞周期和凋亡的影响  

Effects of long-term exposure of NIH/3T3 cells to 1800 MHz electromagnetic radiation on cell cycle and apoptosis

作　　者：侯庆霞[1] 王明连[1] 安广洲[2] 马雪梅[1] 钟儒刚[1] 钟艳艳[1] 刘欢[1] 

机构地区：[1]北京工业大学生命科学与生物工程学院,北京100124 [2]第四军医大学预防医学院辐射医学系

出　　处：《中国预防医学杂志》2014年第3期226-229,共4页Chinese Preventive Medicine

基　　金：北京市教委科技计划面上项目(KM201310005029);北京市教委科技计划重点项目(KZ201110005003)

摘　　要：目的探讨1 800MHz电磁辐射对NIH/3T3细胞周期和凋亡的影响。方法将NIH/3T3细胞假暴露或间断暴露（5min开,10min关）在比吸收率（specific absorption rate,SAR）为2 W/kg,模拟全球移动通信系统（global system for mobile communications,GSM）通话状态信号的1 800MHz电磁场中。每天暴露5h,连续60d,期间每3~4d传代一次。暴露完毕后,用Cell Counting Kit-8（CCK-8）检测细胞活性;染色后用流式细胞仪检测细胞凋亡、细胞周期的变化;用Caspase-3活性检测试剂盒检测细胞凋亡因子Caspase-3的活性变化。结果与假暴露组相比,暴露组细胞活力下降且培养96h后明显下降（t=30.78,P=0.048）;暴露组细胞处于S期（t=3.267,P=0.022）和G2期（t=3.844,P=0.022 9）的百分数较假暴露组有明显增加;细胞凋亡率明显增加（t=3.349,P=0.047）,Caspase-3活性增高（t=13.593,P=0.013）。结论 NIH/3T3细胞经60d1 800MHz模拟手机电磁辐射后,细胞活性下降,S期和G2期阻滞,同时细胞凋亡率增加。Objective To discuss the effect of long term exposure of NIH/3T3cells to 1 800MHz electromagnetic radiation（EMR）on cell cycle and apoptosis. Methods NIH/3T3cells were exposed intermittently（on for 5min and off for 10min）for 5h/d to 1 800MHz EMR at a specific absorption rate（SAR）of 2W/ kg by simulating Global System for Mobile Communications（GSM）Talk for total 60days.Control cells were sham-exposed.Cells were subcultured every 3-4days.CCK-8kit was used to determine the viability of cells and apoptosis and cell cycle were analyzed by flow cytometry.Colorimetric assay was applied for Caspase-3activity detection. Results Compared with control cells,the viability of cells exposed to EMR decreased especially after 96hculture（t=30.78,P=0.048）,while the percentages of cells at S phase（t=3.267,P=0.022） and G2phase（t=3.844,P=0.0229）significantly increased.When exposed to EMR,the percentages of cells in early apoptosis as well as in late apoptosis were significantly higher（t=3.349,P=0.047）,meanwhile,cell Caspase-3activity also increased dramatically（t=13.593,P=0.013）. Conclusions Long term exposure of NIH/3T3cells to 1 800MHz EMR may reduce cell viability,hinder cell cycle,and even trigger cell apoptosis.

关 键 词：电磁辐射 NIH 3T3细胞 细胞周期 细胞凋亡 

分 类 号：R144[医药卫生—公共卫生与预防医学]