阿霉素、5-氟尿嘧啶联合热疗对SMMC-7721细胞增殖及凋亡的影响  

Effect of adriamycin and 5-fluorouracil combined with hyperthermia on proliferation and apoptosis of SMMC-7721 cells

作　　者：张继新[1] 庞志刚[1] 刘超[1] 刘新江[1] 尚闯[1] 耿翔[1] 

机构地区：[1]郑州大学第二附属医院普外科,郑州450014

出　　处：《郑州大学学报（医学版）》2014年第3期365-367,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省医学科技攻关计划项目201303059

摘　　要：目的:探讨阿霉素(ADM)、5-氟尿嘧啶(5-FU)联合热疗对肝癌SMMC-7721细胞增殖和凋亡的影响。方法:SMMC-7721细胞分为对照组、单纯热疗组、5-FU+ADM组、ADM+热疗组、5-FU+热疗组、5-FU+ADM+热疗组,各组热处理均为43℃水浴1 h再37℃培养23 h。各组处理24 h后采用MTT法检测细胞的增殖,计算增殖抑制率,采用流式细胞术检测细胞凋亡,计算凋亡率。结果:5-FU+ADM+热疗组细胞增殖抑制率高于单纯热疗组、5-FU+ADM组、ADM+热疗组、5-FU+热疗组[(74.66±1.61)%vs(19.76±1.41)%、(55.04±1.89)%、(40.21±2.27)%、(35.56±2.24)%,(F=76.843,P<0.001)]。5-FU+ADM+热疗组的细胞凋亡率高于单纯热疗组、5-FU+ADM组、ADM+热疗组、5-FU+热疗组[(80.13±2.42)%vs(28.38±1.92)%、(65.40±2.05)%、(49.32±1.76)%、(45.99±1.70)%,(F=230.506,P<0.001)]。结论:加热能增强5-FU联合ADM化疗对SMMC-7721细胞增殖的抑制和凋亡诱导作用,其效应优于单纯热疗或ADM与5-FU联合以及单纯化疗联合热疗。Aim：To study the effect of adriamycin （ADM） and 5-fluorouracil（5-FU） combined with hyperthermia on proliferation and apoptosis of SMMC-7721 cells.Methods：SMMC-7721 cells were allocated into 6 groups：control group,pure hyperthermia group, 5-FU＋ADM group, ADM＋hyperthermia group, 5-FU＋hyperthermia group, 5-FU＋ADM＋hyperther-mia group.The cell proliferation inhibition effect was evaluated by MTT assay .The apoptosis of SMMC-7721 cells were deter-mined by flow cytometry .Results：The cell proliferation inhibition rate of 5-FU＋ADM＋hyperthermia group was higher than those of pure hyperthermia group, 5-FU＋ADM group, ADM＋hyperthermia group, 5-FU＋hyperthermia group[（74.66 ± 1.61）%vs （19.76 ±1.41）%,（55.04 ±1.89）%,（40.21 ±2.27）%,（35.56 ±2.24）%,（F=76.843,P〈0.001）];the apoptosis rate of 5-FU＋ADM＋hyperthermia group was higher than those of pure hyperthermia group , 5-FU＋ADM group, ADM＋hyperthermia group, 5-FU＋hyperthermia group [（80.13 ±2.42）% vs （28.38 ±1.92）%,（65.40 ±2.05）%, （49.32 ±1.76）%,（45.99 ±1.70）%,（F=230.506,P〈0.001）].Conclusion：ADM and 5-FU combined with hyperther-mia can increase the inhibition effect on proliferation and induce apoptosis of SMMC-7721 cells compared with pure hyperther-mia, or ADM and 5-FU combination or single chemotherapy combined with hyperthermia .

关 键 词：阿霉素 5-氟尿嘧啶 热疗 热化疗 SMMC-7721细胞 细胞增殖 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤]